LABELLING OF DNA AND CELL DIVISION IN SO CALLED NON-DIVIDING TISSUES
Open Access
- 1 July 1964
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 22 (1), 21-28
- https://doi.org/10.1083/jcb.22.1.21
Abstract
Autoradiographs of adult mice killed at various times after injection of tritiated thymidine show significant numbers of labelled nuclei in organs in which mitoses are either very rare or completely absent. The proportion of labelled cells that divide was estimated from the decline in the number of grains per nucleus, the number of pairs of labelled cells in sheets of epithelium in squashes, the number of labelled metaphases after 6 hours'' treatment with Colcemid, and the ratio of mitotic index to labelling index. The longest possible duration of G2 in the epithelial cells of seminal vesicles was deduced from the results of Feulgen photometry. The results show that only a small proportion of the labelled cells divide in the seminal vesicles and liver, while probably none divide in brain, smooth muscle, and heart muscle. It is suggested that, in the so called "non-dividing tissues" of adult mice, cells periodically renew their DNA by a process the details of which are as yet unknown.Keywords
This publication has 7 references indexed in Scilit:
- Loss of Radioactivity from Labeled DNA of Primary Human Amnion CellsScience, 1963
- Studies on the Incorporation of Tritiated Thymidine in Desoxyribonucleic Acid in Mouse Tissues and on Its Radiation EffectsInternational Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine, 1963
- Adenosine Triphosphatase Activity of Glycerinated Muscle Fibres in a Mechanical FieldNature, 1963
- Cell proliferation and migration as revealed by radioautography after injection of thymidine‐H3 into male rats and miceJournal of Anatomy, 1960
- Autoradiographic Investigation of Incorporation of H 3 -Thymidine into Cells of the Rat and MouseScience, 1960
- CATABOLISM OF TISSUE NUCLEIC ACID IN THE RAT .1. REPLACEMENT TIME OF DEOXYRIBONUCLEIC ACID1960
- Incorporation of Labelled Thymidine in the Seminal Vesicle of the MouseNature, 1959