Fast screening of highly glycosylated plant sphingolipids by tandem mass spectrometry
- 15 September 2011
- journal article
- research article
- Published by Wiley in Rapid Communications in Mass Spectrometry
- Vol. 25 (20), 3131-3145
- https://doi.org/10.1002/rcm.5206
Abstract
The structural characterization of Glycosyl-Inositol-Phospho-Ceramides (GIPCs), which are the main sphingolipids of plant tissues, is a critical step towards the understanding of their physiological function. After optimization of their extraction, numerous plant GIPCs have been characterized by mass spectrometry. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) full scan analysis of negative ions provides a quick overview of GIPC distribution. Clear differences were observed for the two plant models studied: six GIPC series bearing from two to seven saccharide units were detected in tobacco BY-2 cell extracts, whereas GIPCs extracted from A. thaliana cell cultures and leaves were less diverse, with a dominance of species containing only two saccharide units. The number of GIPC species was around 50 in A. thaliana and 120 in tobacco BY-2 cells. MALDI-MS/MS spectra gave access to detailed structural information relative to the ceramide moiety, the polar head, as well as the number and types of saccharide units. Once released from GIPCs, fatty acid chains and long-chain bases were analyzed by GC/MS to verify that all GIPC series were taken into account by the MALDI-MS/MS approach. ESI-MS/MS provided complementary information for the identification of isobaric species and fatty acid chains. Such a methodology, mostly relying on MALDI-MS/MS, should open new avenues to determine structure-function relationships between glycosphingolipids and membrane organization. Copyright © 2011 John Wiley & Sons, Ltd.Keywords
This publication has 24 references indexed in Scilit:
- PAMP (Pathogen-associated Molecular Pattern)-induced Changes in Plasma Membrane Compartmentalization Reveal Novel Components of Plant ImmunityJournal of Biological Chemistry, 2010
- Localization and imaging of sialylated glycosphingolipids in brain tissue sections by MALDI mass spectrometryGlycobiology, 2010
- Plant sphingolipids: decoding the enigma of the SphinxNew Phytologist, 2009
- Quantitative Proteomics Reveals a Dynamic Association of Proteins to Detergent-resistant Membranes upon Elicitor Signaling in TobaccoMolecular & Cellular Proteomics, 2009
- Plasmodesmata in Arabidopsis thaliana suspension cellsProtoplasma, 2004
- Disruption of theFATBGene in Arabidopsis Demonstrates an Essential Role of Saturated Fatty Acids in Plant GrowthPlant Cell, 2003
- Comparative analysis of glycosylinositol phosphorylceramides from fungi by electrospray tandem mass spectrometry with low-energy collision-induced dissociation of Li+ adduct ionsRapid Communications in Mass Spectrometry, 2001
- Differential Expression of a Glycosyl Inositol Phospholipid Antigen on the Peribacteroid Membrane during Pea Nodule DevelopmentMolecular Plant-Microbe Interactions®, 1995
- Structure of a major glycophosphoceramide from tobacco leaves, PSL-I: 2-deoxy-2-acetamido-D-glucopyranosyl(α1 → 4)-D-glucuronopyranosyl(α1 → 2)myoinositol-1-O-phosphoceramideBiochemistry, 1978
- Characterization of Inositol-containing Phosphosphingolipids from Tobacco LeavesPlant Physiology, 1975