Kappa-casein genotyping in tropical milking Criollo and its association to milk production and composition
- 1 November 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in Tropical Animal Health and Production
- Vol. 52 (6), 3885-3888
- https://doi.org/10.1007/s11250-020-02317-3
Abstract
Caseins are abundant proteins in milk and found in four types (alpha(S1), alpha(S2), beta, and kappa). There is substantial variation in the allelic and genotypic frequencies of the kappa-casein gene in different cattle breeds, although the tropical milking Criollo (TMC) has not yet been investigated. The aim was to determine the allelic and genotypic frequencies in the kappa-casein gene for two alleles (A and B) in TMC and further investigate its association to milk production and composition. A total of 180 TMC females were genotyped from blood samples. kappa-Casein genotyping was performed using restriction fragment length polymorphism (RFLP) after polymerase chain reaction (PCR)-based amplification of genomic DNA. Allele frequencies were 0.39 for A-allele and 0.61 for B-allele (P < 0.05). Genotype frequencies were 0.09 (AA), 0.60 (AB), and 0.31 (BB) (P < 0.05). The kappa-casein genotype in TMC cows did not affect milk yield or composition. In sum, the TMC has high frequencies of the B-allele and AB/BB genotypes, although there are no association of such genotypes and milk traits.Keywords
Funding Information
- CONACyT
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