Tryptamine-4,5-dione, a Putative Endotoxic Metabolite of the Superoxide-Mediated Oxidation of Serotonin, Is a Mitochondrial Toxin: Possible Implications in Neurodegenerative Brain Disorders
- 10 April 1999
- journal article
- Published by American Chemical Society (ACS) in Chemical Research in Toxicology
- Vol. 12 (5), 429-436
- https://doi.org/10.1021/tx9801615
Abstract
The release and subsequent reuptake of 5-hydroxytryptamine (5-HT) and cytoplasmic superoxide (O2-•) generation have both been implicated as important factors associated with the degeneration of serotonergic neurons evoked by methamphetamine (MA) and cerebral ischemia-reperfusion (I-R). Such observations raise the possibility that tryptamine-4,5-dione (T-4,5-D), the major in vitro product of the O2-•-mediated oxidation of 5-HT, might be an endotoxicant that contributes to serotonergic neurodegeneration. When incubated with intact rat brain mitochondria, T-4,5-D (≤100 μM) uncouples respiration and inhibits state 3. Experiments with rat brain mitochondrial membrane preparations confirm that T-4,5-D evokes irreversible inhibition of NADH-coenzyme Q1 (CoQ1) reductase and cytochrome c oxidase (COX) apparently by covalently modifying key sulfhydryl (SH) residues at or close to the active sites of these respiratory enzyme complexes. Ascorbic acid blocks the inhibition of NADH-CoQ1 reductase by maintaining T-4,5-D predominantly as 4,5-dihydroxytryptamine (4,5-DHT), thus preventing its reaction with SH residues. In contrast, ascorbic acid potentiates the irreversible inhibition of COX by T-4,5-D. This may be because the T-4,5-D−4,5-DHT couple redox cycles in the presence of excess ascorbate and molecular oxygen to cogenerate O2-• and H2O2 that together react with trace levels of iron to form an oxo−iron complex that selectively damages COX. Thus, T-4,5-D might be an endotoxicant that, dependent on intraneuronal conditions, mediates irreversible damage to mitochondrial respiratory enzyme complexes and contributes to the serotonergic neurodegeneration evoked by MA and I-R.Keywords
This publication has 21 references indexed in Scilit:
- Lack of involvement of glutamate‐induced excitotoxicity in MPP+ toxicity in striatal dopaminergic terminals: possible involvement of ascorbateBritish Journal of Pharmacology, 1997
- Oxidative Stress in Neurodegenerative DiseasesAnnual Review of Pharmacology and Toxicology, 1996
- Regional Differences in the Ontogeny of the Serotonergic Projection to the Cerebral CortexExperimental Neurology, 1996
- Hydroxyl Radical‐Mediated Oxidation of Serotonin: Potential Insights into the Neurotoxicity of MethamphetamineJournal of Neurochemistry, 1995
- Rapid ATP Loss Caused by Methamphetamine in the Mouse Striatum: Relationship Between Energy Impairment and Dopaminergic NeurotoxicityJournal of Neurochemistry, 1994
- Delayed Onset of Neurologic Deterioration following Anoxia/Ischemia Coincides with Appearance of Impaired Brain Mitochondrial Respiration and Decreased Cytochrome Oxidase ActivityJournal of Cerebral Blood Flow & Metabolism, 1990
- Acute inactivation of tryptophan hydroxylase by amphetamine analogs involves the oxidation of sulfhydryl sitesEuropean Journal of Pharmacology: Molecular Pharmacology, 1989
- Elevation of the Extracellular Concentrations of Glutamate and Aspartate in Rat Hippocampus During Transient Cerebral Ischemia Monitored by Intracerebral MicrodialysisJournal of Neurochemistry, 1984
- Regional Energy Balance in Rat Brain After Transient Forebrain IschemiaJournal of Neurochemistry, 1983
- Implication of SH‐groups in the mitochondrial energy‐coupling system revealed by measurements of 14C‐ethacrynate incorporation into rat liver mitochondriaFEBS Letters, 1971