Severe Aplastic Anaemia: Correlation of in Vitro Tests with Clinical Response to Immunosuppression in 20 Patients
- 1 March 1981
- journal article
- research article
- Published by Wiley in British Journal of Haematology
- Vol. 47 (3), 423-433
- https://doi.org/10.1111/j.1365-2141.1981.tb02810.x
Abstract
Summary. Colony formation in agar (CFU-c) was studied in 20 patients with severe aplastic anaemia by three different assays: (1) cultures of light density untreated marrow cells; (2) cultures of marrow cells manipulated in order to enhance colony formation (pretreatment with antilymphocytic globulin, ALG, or 6-methylprednisolone, 6-MPr, T cell depletion, adherent cell (AC) depletion, depletion of both AC and T cells), and (3) co-culture of putative suppressor T cells with autologous T-depleted marrow cells. By the first assay, all patients showed poor colony formation (1 1.5 colonies/105 cells; normal controls 46.18 colonies/105 cells). By the second assay, ALG and 6-MPr had no significant effect on colony formation. Removal of adherent cells proved equally without effect on colony growth. On the contrary, removal of T cells enhanced significantly (P < 0.001) colony formation in 10 out of 20 patients. By the third assay, colony formation of marrow cells (deprived of T lymphocytes) was inhibited by the addition of autologous T cells in six patients studied. All patients were given high dose bolus 6-MPr as first treatment on admission: only patients who had detectable suppressor T cells in their marrow achieved a complete autologous haematologic reconstitution after 6-MPr or after 6-MPr and ALG. The results of this study indicate that detection of CFU-c/suppressor T cells correlates with responses to immunosuppressive regimens, and may thus help to identify patients with immune mediated aplastic anaemia.This publication has 18 references indexed in Scilit:
- A survey of the effectiveness of cyclophosphamide in patients with severe aplastic anemiaAmerican Journal of Hematology, 1980
- Lymphoid antigens (LY) on leukaemic cell populations: Recognition by means of antilymphocytic globulins and clinical implicationsLeukemia Research, 1979
- Effect of Antilymphocyte Globulin on Granulocyte Precursors in Aplastic AnaemiaBritish Journal of Haematology, 1979
- Circulating Suppressor Cells in Aplastic AnemiaVox Sanguinis, 1979
- The Contribution of in Vitro Cultures to the Elucidation of the Pathogenesis of Aplastic AnaemiaPublished by Springer Science and Business Media LLC ,1979
- Antilymphocyte Globulin Treatment in Severe Aplastic Anemia — Comparison with Bone Marrow Transplantation. Report of 60 CasesPublished by Springer Science and Business Media LLC ,1979
- Bone Marrow Transplantation for Severe Aplastic AnemiaActa Haematologica, 1979
- TREATMENT OF APLASTIC ANÆMIA BY ANTILYMPHOCYTE GLOBULIN WITH AND WITHOUT ALLOGENEIC BONE-MARROW INFUSIONSThe Lancet, 1977
- APLASTIC ANÆMIA: EVIDENCE FOR AN IMMUNOLOGICAL MECHANISMThe Lancet, 1976
- Human bone marrow colony growth in agar‐gelJournal of Cellular Physiology, 1970