Further Characterization of the Three Polypeptide Chains of Bovine and Human Short‐Chain Collagen (Intima Collagen)
- 1 June 1983
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 133 (1), 39-46
- https://doi.org/10.1111/j.1432-1033.1983.tb07427.x
Abstract
Short-chain collagen was isolated from bovine and from human placenta after limited pepsin digestion. By reductive cleavage of disulfide bonds under non-denaturing conditions, mainly non-collagenous domains were cleaved off yielding a uniform component composed of 3 polypeptide chains with molecular masses between 37 kDa [kilodalton] and 48 kDa in a nearly equimolar ratio. The chains (SC1, SC2 and SC3) were isolated and characterized. By comparison of peptide patterns obtained after various cleavage procedures, they could be identified as more or less shortened forms of SC1, SC2 and SC3, the isolation of which from bovine short-chain collagen has been described. The peptide patterns, as well as N-terminal sequence determination, give evidence for the genetic individuality of the 3 chains; the data so far available suggest that together they form 1 triple-helical structure which represents the collagenous domain of the basic molecular unit of short-chain collagen.This publication has 12 references indexed in Scilit:
- Isolation and native characterization of cysteine-rich collagens from bovine placental tissues and uterus and their relationship to types IV and V collagensBioscience Reports, 1982
- Covalent Crosslinking between Molecules of Type I and Type III CollagenEuropean Journal of Biochemistry, 1982
- Minor collagens of chicken hyaline cartilageBiochemistry, 1981
- Macromolecular structure of basement membrane collagensFEBS Letters, 1981
- Characterization of a unique collagenous fraction from limited pepsin digests of human placental tissue: molecular organization of the native aggregateBiochemistry, 1981
- A Cysteine‐Rich Collagenous Protein from Bovine PlacentaEuropean Journal of Biochemistry, 1981
- Two-dimensional CNBr peptide patterns of collagen types I, II and IIICollagen and Related Research, 1980
- Isolation of a macromolecular collagenousfraction and AB2 collagen from calf skinFEBS Letters, 1980
- Isolation of three collagenous components of probable basement membrane origin from several tissuesBiochemical and Biophysical Research Communications, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970