Conjugational Fertility and Location of Chloramphenicol Biosynthesis Genes on the Chromosomal Linkage Map of Streptomyces venezuelae
Open Access
- 1 May 1986
- journal article
- research article
- Published by Microbiology Society in Microbiology
- Vol. 132 (5), 1327-1338
- https://doi.org/10.1099/00221287-132-5-1327
Abstract
Summary: In Streptomyces venezuelae fertility, defined as chromosomal gene recombination, was enhanced over 1000-fold when one parent in a biparental conjugational cross lacked the physicallyundetected plasmid SVP1, as compared with crosses in which both parents carried SVP1. The existence of SVP1 and at least two other fertility plasmids, SVP2 and SVP3, was detected in S. venezuelae by ‘lethal zygosis’ elicited by a plasmid-plus mycelium in contact with a plasmid-minus mycelium. Conjugational crosses were used to construct a linkage map of S. venezuelae which was highly consistent with the map of analogous loci in S. coelicolor A3(2). A cluster of genes governing chloramphenicol biosynthesis was located near arg, cys and pdxB genes at a position roughly equivalent to the 1–2 o'clock region of the S. coelicolor A3(2) map.Keywords
This publication has 9 references indexed in Scilit:
- Genetic analysis of erythromycin production in Streptomyces erythreusJournal of Bacteriology, 1985
- Isolation and Characterization of Streptomyces venezuelae Mutants Blocked in Chloramphenicol BiosynthesisMicrobiology, 1985
- Localized hydroxylamine mutagenesis, and cotransduction of threonine and lysine genes, in Streptomyces venezuelaeJournal of Bacteriology, 1983
- Plasmids, Recombination and Chromosome Mapping in Streptomyces lividans 66Microbiology, 1983
- Evidence for a chromosomal location of the genes coding for chloramphenicol production in Streptomyces venezuelaeJournal of Bacteriology, 1983
- Excision of chromosomal DNA sequences from Streptomyces coelicolor forms a novel family of plasmids detectable in Streptomyces lividansMolecular Genetics and Genomics, 1981
- Genetics and biochemical studies of chloramphenicol-nonproducing mutants of Streptomyces venezuelae carrying plasmid.The Journal of Antibiotics, 1979
- Genetic Evidence for a Plasmid Controlling Fertility in an Industrial Strain of Streptomyces rimosusJournal of General Microbiology, 1978
- A Plasmid Involved in Chloramphenicol Production in Streptomyces venezuelae: Evidence from Genetic MappingJournal of General Microbiology, 1975