Extracellular matrix of lymphoid tissues in the chick.
Open Access
- 1 May 1989
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 37 (5), 757-763
- https://doi.org/10.1177/37.5.2703709
Abstract
We describe the immunohistochemical distribution of components of the extracellular matrix of the chick lymphoid system. In the thymus, basement membranes of epithelial cells bordering the lobules were intensely stained by laminin antibodies; fibronectin antibodies labeled the capsule and the septal matrix, and similar reactivity was seen with tropoelastin and gp 115 antibodies. No positivity was detected with any of the antibodies within the cortical parenchymal cells. Laminin was not detected in the medullary parenchyma, whereas fibronectin was present as coarse fibers. Tropoelastin and gp 115 appeared as a finer and more diffuse meshwork. In the bursa, laminin antibodies outlined the epithelial cells separating the cortex from the medulla. Fibronectin, tropoelastin, and gp 115 antibody stained the interfollicular septa and the cortical matrix, although to a different extent. Laminin was also detected in association with the interfollicular epithelium (IFE) basement membrane, whereas no staining was found underneath the follicle-associated epithelium (FAE). FAE cells not only lack a proper basement membrane but are also not separated from medullary lymphocytes by any of the other extracellular matrix components were investigated. Consequently, medullary lymphocytes are not sequestered, and can come easily into contact with antigens present in the intestinal lumen. All four antibodies stained the spleen capsule and spleen blood vessels, tropoelastin and gp 115 antibodies giving the strongest reactivity. A fine trabecular staining pattern was detected with gp 115 antibodies in the white pulp.This publication has 20 references indexed in Scilit:
- Monoclonal antibodies to laminin reveal the heterogeneity of basement membranes in the developing and adult mouse tissues.The Journal of cell biology, 1984
- MONOCLONAL-ANTIBODY TO TYPE-IV COLLAGEN WITH SELECTIVE BASEMENT-MEMBRANE LOCALIZATION1984
- Isolation and characterization of a 115,000-dalton matrix-associated glycoprotein from chick aorta.Journal of Biological Chemistry, 1983
- Elastic and precursor fibres in the normal human eyeExperimental Eye Research, 1983
- Domain and basement membrane specificity of a monoclonal antibody against chicken type IV collagen.The Journal of cell biology, 1982
- Elastic fibers associated with collagenous fibrils surrounded by reticular cells in lymph nodes of the rat as revealed by electron microscopy after orcein stainingCell and tissue research, 1981
- IMMUNOHISTOCHEMICAL LOCALIZATION OF TYPES I, III AND IV (BASEMENT MEMBRANE) COLLAGENS IN THE LYMPH NODE: CO-DISTRIBUTION OF TYPES I AND III COLLAGENS IN THE RETICULAR FIBERS Biomedical Research, 1981
- Laminin–a glycoprotein from basement membranes.Journal of Biological Chemistry, 1979
- Binding of soluble form of fibroblast surface protein, fibronectin, to collagenInternational Journal of Cancer, 1977
- Synthesis of elastin in aortas from chick embryos. Conversion of newly secreted elastin to cross-linked elastin without apparent proteolysis of the moleculeBiochemistry, 1977