In situ sequencing for RNA analysis in preserved tissue and cells
- 14 July 2013
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Methods
- Vol. 10 (9), 857-860
- https://doi.org/10.1038/nmeth.2563
Abstract
Tissue gene expression profiling is performed on homogenates or on populations of isolated single cells to resolve molecular states of different cell types. In both approaches, histological context is lost. We have developed an in situ sequencing method for parallel targeted analysis of short RNA fragments in morphologically preserved cells and tissue. We demonstrate in situ sequencing of point mutations and multiplexed gene expression profiling in human breast cancer tissue sections.Keywords
This publication has 17 references indexed in Scilit:
- Single-cell dissection of transcriptional heterogeneity in human colon tumorsNature Biotechnology, 2011
- Tumour evolution inferred by single-cell sequencingNature, 2011
- In situ detection and genotyping of individual mRNA moleculesNature Methods, 2010
- mRNA-Seq whole-transcriptome analysis of a single cellNature Methods, 2009
- RNA-Seq: a revolutionary tool for transcriptomicsNature Reviews Genetics, 2009
- Development of the 21-Gene Assay and Its Application in Clinical Practice and Clinical TrialsJournal of Clinical Oncology, 2008
- Gene expression and the myth of the average cellTrends in Cell Biology, 2003
- Signal amplification of padlock probes by rolling circle replicationNucleic Acids Research, 1998
- A pyramid approach to subpixel registration based on intensityIEEE Transactions on Image Processing, 1998
- Padlock Probes: Circularizing Oligonucleotides for Localized DNA DetectionScience, 1994