Phagocytic and oxidative burst activity of neutrophils in the end stage of liver cirrhosis
Open Access
- 1 January 2005
- journal article
- Published by Baishideng Publishing Group Inc. in World Journal of Gastroenterology
- Vol. 11 (48), 7661-7665
- https://doi.org/10.3748/wjg.v11.i48.7661
Abstract
AIM: To evaluate the phagocytic activity and neutrophil oxidative burst in liver cirrhosis. METHODS: In 45 patients with advanced postalcoholic liver cirrhosis (aged 45±14 years) and in 25 healthy volunteers (aged 38±5 years), the percentage of phagocytizing cells after in vitro incubation with E. coli (Phagotest Kit), phagocytic activity (mean intensity of fluorescence, MIF) and the percentage of neutrophil oxidative burst (Bursttest Kit), and the level of free oxygen radical production (MIF of Rodamine 123) were analyzed by flow cytometry. The levels of soluble sICAM-1, sVCAM-1, sP-selectin, sE-selectin, sL-selectin, and TNF-α were determined in blood serum. RESULTS: The percentage of E. coli phagocytizing neutrophils in liver cirrhosis patients was comparable to that in healthy subjects. MIF of neutrophil - ingested E. coli was higher in patients with liver cirrhosis. The oxidative burst in E. coli phagocytizing neutrophils generated less amount of active oxygen compounds in liver cirrhosis patients (MIF of R123: 24.7±7.1 and 29.7±6.6 in healthy, Pvs 50.2±13.3, Pr -0.35, Pr-0.4, Pr-0.45, Pr-0.41, Pr-0.39, P<0.05, respectively). CONCLUSION: Neutrophil metabolic activity diminishes together with the intensification of liver failure. The metabolic potential of phagocytizing neutrophils is significantly lower in liver cirrhosis patients, which can be one of the causes of immune mechanism damage. The evaluation of oxygen metabolism of E. coli-stimulated neutrophils reveals that the amount of released oxygen metabolites is smaller in liver cirrhosis patients than in healthy subjects.Keywords
This publication has 27 references indexed in Scilit:
- LSP1 is an endothelial gatekeeper of leukocyte transendothelial migrationThe Journal of Experimental Medicine, 2005
- Novel Chemokine Responsiveness and Mobilization of Neutrophils during SepsisThe American Journal of Pathology, 2004
- Chemokines in the Pathogenesis of Vascular DiseaseCirculation Research, 2004
- In Vivo Neutrophil Dysfunction in Cirrhotic Patients with Advanced Liver DiseaseThe Journal of Infectious Diseases, 2000
- SEQUESTRATION OF NEUTROPHILS IN THE HEPATIC VASCULATURE DURING ENDOTOXEMIA IS INDEPENDENT OF β2 INTEGRINS AND INTERCELLULAR ADHESION MOLECULE-1Shock, 1996
- Expression of adhesion molecules and their ligands in liver allografts during cytomegalovirus (CMV) infection and acute rejectionTransplant International, 1996
- Plasma interleukin‐8 levels in patients with post‐hepatitic cirrhosis: Relationship to severity of liver disease, portal hypertension and hyperdynamic circulationJournal of Gastroenterology and Hepatology, 1996
- Translocation of gut bacteria in rats with cirrhosis to mesenteric lymph nodes partially explains the pathogenesis of spontaneous bacterial peritonitisJournal of Hepatology, 1994
- Abnormalities of neutrophil phagocytosis, intracellular killing and metabolic activity in alcoholic cirrhosis and hepatitisHepatology, 1986
- Transection of the oesophagus for bleeding oesophageal varicesBritish Journal of Surgery, 1973