Flow cytometric DNA content in myelodysplastic syndromes
Open Access
- 1 November 1983
- Vol. 4 (3), 238-243
- https://doi.org/10.1002/cyto.990040308
Abstract
DNA flow cytometric analysis of unfixed bone marrow cells stained with propidium iodide was carried out in 33 patients with untreated primary myelodysplastic syndromes. Patients with stable clinical course for up to 3 years had higher fractions of cells in S and G2 phases (22.7 ± 12.4% and 12 ± 3.6%) than those who developed acute leukemia and/or died early in the course of disease (14.4 ± 8.5% and 6.6 ± 4%). Median survival was more than 36 mo in patients with S+G2 cell fraction higher than 24%, and 14 mo in the remaining 16 patients with lower values (P < 0.01). Analyses repeated after 3–24 mo showed no major changes in cell proliferation pattern in ten out of 11 patients. The remaining patient had sharp decrease in S and G2 cell fraction 3 mo before the transition into acute leukemia. The DNA index (DI) of bone marrow cells was calculated to assess ploidy. However, comparative evaluation of cytologic, cytogenetic, and flow cytometric data suggest that, under our experimental conditions, the DI may be influenced by factors such as the degree of chromatin compactness.Keywords
This publication has 24 references indexed in Scilit:
- A flow cytometric study of the propidium iodide staining kinetics of human leukocytes and its relationship with chromatin structureCytometry, 1983
- Sequential vincristine, arabinosylcytosine and adriamycin in acute leukemia: Cytologic and cytokinetic studiesCytometry, 1982
- Cell kinetics in acute leukemia. A critical reevaluation based on new dataCancer, 1982
- Proposals for the classification of the myelodysplastic syndromesBritish Journal of Haematology, 1982
- Sideroblastic anemia and development of leukemiaBlut: Zeitschrift für die Gesamte Blutforschung, 1981
- Bone marrow cell kinetics and culture in chronic and subacute myelomonocytic leukemia. Physiopathological interpretation and prognostic importanceLeukemia Research, 1980
- The G0-G1 transition of WI38 cellsExperimental Cell Research, 1977
- Method for the quantitative evaluation of data from flow microfluorometryComputers and Biomedical Research, 1976
- Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining.The Journal of cell biology, 1975
- Preleukemia: The hematologie syndrome preceding acute leukemiaAmerican Journal Of Medicine, 1973