Identification of Components Associated with Thermal Acclimation of Photosystem II in Synechocystis sp. PCC6803
Open Access
- 6 May 2010
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 5 (5), e10511
- https://doi.org/10.1371/journal.pone.0010511
Abstract
Photosystem II (PSII) is the most thermally sensitive component of photosynthesis. Thermal acclimation of this complex activity is likely to be critically important to the ability of photosynthetic organisms to tolerate temperature changes in the environment. We have analysed gene expression using whole-genome microarrays and monitored alterations in physiology during acclimation of PSII to elevated growth temperature in Synechocystis sp. PCC 6803. PSII acclimation is complete within 480 minutes of exposure to elevated temperature and is associated with a highly dynamic transcriptional response. 176 genes were identified and classified into seven distinct response profile groups. Response profiles suggest the existence of an early transient phase and a sustained phase to the acclimation response. The early phase was characterised by induction of general stress response genes, including heat shock proteins, which are likely to influence PSII thermal stability. The sustained phase consisted of acclimation-specific alterations that are involved in other cellular processes. Sustained responses included genes involved in phycobillisome structure and modification, photosynthesis, respiration, lipid metabolism and motility. Approximately 60% of genes with sustained altered expression levels have no known function. The potential role of differentially expressed genes in thermotolerance and acclimation is discussed. We have characterised the acclimation physiology of selected gene ‘knockouts’ to elucidate possible gene function in the response. All mutants show lower PSII rates under normal growth conditions. Basal PSII thermotolerance was affected by mutations in clpB1, cpcC2, hspA, htpG and slr1674. Final PSII thermotolerance was affected by mutations in cpcC2, hik34, hspA and hypA1, suggesting that these gene products play roles in long-term thermal acclimation of PSII.Keywords
This publication has 64 references indexed in Scilit:
- Quality Control of Photosystem IIJournal of Biological Chemistry, 2008
- Core Genome Responses Involved in Acclimation to High TemperaturePlant Physiology, 2007
- The Membrane-Associated CpcG2-Phycobilisome in Synechocystis: A New Photosystem I AntennaPlant Physiology, 2007
- Light-Induced Energy Dissipation in Iron-Starved Cyanobacteria: Roles of OCP and IsiA ProteinsPlant Cell, 2007
- The SigB σ factor mediates high‐temperature responses in the cyanobacterium Synechocystis sp. PCC6803FEBS Letters, 2005
- Gene Expression Omnibus: NCBI gene expression and hybridization array data repositoryNucleic Acids Research, 2002
- Salt Stress and Hyperosmotic Stress Regulate the Expression of Different Sets of Genes in Synechocystis sp. PCC 6803Biochemical and Biophysical Research Communications, 2002
- Sequence Analysis of the Genome of the Unicellular Cyanobacterium Synechocystis sp. Strain PCC6803. II. Sequence Determination of the Entire Genome and Assignment of Potential Protein-coding RegionsDNA Research, 1996
- The birA gene of Escherichia coli encodes a biotin holoenzyme synthetaseJournal of Molecular Biology, 1981
- Sites of heat sensitivity in chloroplasts and differential inactivation of cyclic and noncyclic photophosphorylation by heatingJournal of Thermal Biology, 1976