Norepinephrine-stimulated hypertrophy of cultured rat myocardial cells is an alpha 1 adrenergic response.
Open Access
- 1 August 1983
- journal article
- Published by American Society for Clinical Investigation in JCI Insight
- Vol. 72 (2), 732-738
- https://doi.org/10.1172/jci111023
Abstract
We have shown recently that norepinephrine stimulates muscle cell hypertrophy in primary cultures from the neonatal rat ventricle and that this stimulation is not blocked by the beta adrenergic antagonist propranolol. The present study was done to define the adrenergic specificity of the myocyte hypertrophic response to norepinephrine. 90% pure, single-cell cultures of nongrowing myocytes were maintained in serum-free medium 199 with transferin and insulin. Myocyte size was quantitated 48 h after addition of adrenergic agents, by measuring cell volume, cell surface area, and cell protein. L-norepinephrine increased myocyte size to a maximum 150% of control; half-maximum effect was obtained at a concentration of 0.2 microM. This increase in cell size was inhibited by the nonselective alpha adrenergic antagonist phentolamine and by the alpha 1 adrenergic antagonists prazosin and terazosin; it was not inhibited by propranolol or by the alpha 2 adrenergic antagonist yohimbine. The beta adrenergic agonist isoproterenol did not increase cell size. Thus, norepinephrine-stimulated hypertrophy of cultured rat myocardial cells is an alpha 1 adrenergic response.Keywords
This publication has 16 references indexed in Scilit:
- Differentiation of rat myocytes in single cell cultures with and without proliferating nonmyocardial cells. Cross-striations, ultrastructure, and chronotropic response to isoproterenol.Circulation Research, 1982
- Cardiac Sympathetic Nerves as the Final Common Pathway in the Induction of Adaptive Cardiac HypertrophyClinical Science, 1981
- Studies on beta-adrenoceptor mediated facilitation of sympathetic neurotransmission.1981
- Presynaptic regulation of the release of catecholamines.1980
- Alpha1-adrenergic receptors in guinea pig myocardium: Identification by binding of a new radioligand, (3H)-prazosinBiochemical and Biophysical Research Communications, 1979
- Metabolic and cardiovascular responses to prolonged noradrenaline load and their antagonism by beta blockade in the ratActa Physiologica Scandinavica, 1978
- Norepinephrine and the presynaptic control of adrenergic transmitter release.1978
- ALPHA ADRENERGIC INVOLVEMENT IN HEART METABOLISM - EFFECTS ON ADENOSINE CYCLIC 3',5'-MONOPHOSPHATE, ADENOSINE CYCLIC 3',5'-MONOPHOSPHATE-DEPENDENT PROTEIN-KINASE, GUANOSINE CYCLIC 3',5'-MONOPHOSPHATE, AND GLUCOSE-TRANSPORT1977
- Adrenergic antagonists, and a possible link between the increase in cyclic adenosine 3′,5′‐monophosphate and DNA synthesis during liver regenerationJournal of Cellular Physiology, 1973
- Studies on isoproterenol-induced cardiomegaly in ratsAmerican Heart Journal, 1969