Use of diploid and triploid trout erythrocytes as internal standards in flow cytometry
- 1 January 1991
- Vol. 12 (3), 275-278
- https://doi.org/10.1002/cyto.990120311
Abstract
DNA content determination requires the use of standards. Vindelov has shown the need to use two standards. Chicken and trout erythrocytes are commonly used, but they are not ideal standards. On the one hand, their DNA contents rarely frame the studied sample DNA content, and, on the other hand, as their base compositions are different in terms of A+T/G+C, their relative indices change according to the stains used. Use of triploid trout erythrocytes instead of chicken erythrocytes allows elimination of these two drawbacks; however, diploid trout must be differentiated from triploid trout. The present paper shows that an anatomic malformation is found with the triploid trout and so justifies the use of paired diploid and triploid trout as standards to measure nuclear DNA content.Keywords
This publication has 7 references indexed in Scilit:
- Reference standards for flow cytometry and application in comparative studies of nuclear DNA contentCytometry, 1989
- Flow cytometric analysis of DNA content differences in blood samples obtained by leucoconcentrationCytometry, 1988
- Technique and staining optimization leucoconcentrationCytometry, 1987
- Trout and salmon erythrocytes and human leukocytes as internal standards for ploidy control in flow cytometryCytometry, 1987
- The use of trout erythrocytes and human lymphocytes for standardization in flow cytometryCytometry, 1983
- Standardization of high‐resolution flow cytometric DNA analysis by the simultaneous use of chicken and trout red blood cells as internal reference standardsCytometry, 1983
- Flow-cytogenetics: sources of DNA content variation among euploid individualsCytogenetic and Genome Research, 1977