Cotransfer of two linked human genes into cultured mouse cells.
- 1 April 1976
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 73 (4), 1274-1278
- https://doi.org/10.1073/pnas.73.4.1274
Abstract
Two linked human genes which code for the expression of cytosol thymidine kinase (ATP:thymidine 5'-phosphotransferase, EC 2.7.1.75) and galactokinase (ATP:D-galactose 1-phosphotransferase, EC 2.7.1.6) have been cotransferred via purified metaphase chromosomes from the human lymphoblastoid cell line WI-L2a, into mouse L-cells [B82 and LM(TK-)]. Both genes have previously been shown to be closely linked on the human chromosome E17, band q21-22. Coexpression of both human enzyme markers was detected in two out of eight gene transfer clones, whilst the remaining six clones contained only human cytosol thymidine kinase, as shown by electrophoretic techniques. A further 23 human enzyme markers corresponding to 15 different human chromosomes were found to be absent in these gene transfer clones. No human chromosome or chromosomal fragment could be detected by karyotype analyses. Some of the gene transfer clones rapidly lost the transferred donor material when grown in nonselective medium, whereas others expressed a stable phenotype under these conditions. Prolonged maintenance in selective medium favors the survival of gene transfer cells expressing a stable phenotype. Possibly these cells harbor the donor genes integrated into a recipient chromosome.Keywords
This publication has 25 references indexed in Scilit:
- A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and virusesNature, 1975
- ACQUISITION OF CHICK CYTOSOL THYMIDINE KINASE ACTIVITY BY THYMIDINE KINASE-DEFICIENT MOUSE FIBROBLAST CELLS AFTER FUSION WITH CHICK ERYTHROCYTESThe Journal of cell biology, 1974
- Assignment of the gene for galactokinase to human chromosome 17 and its regional localisation to band q21-22Nature, 1974
- A new electrophoretic technique for mouse, human, and Chinese hamster galactokinaseCytogenetic and Genome Research, 1974
- A REVIEW OF ENZYME POLYMORPHISM, LINKAGE AND ELECTROPHORETIC CONDITIONS FOR MOUSE AND SOMATIC CELL HYBRIDS IN STARCH GELSJournal of Histochemistry & Cytochemistry, 1973
- Quinacrine fluorescent karyotypes of human diploid and heteroploid cell linesCytogenetic and Genome Research, 1971
- Factors influencing the “effective mating rate” of mammalian cellsExperimental Cell Research, 1970
- The use of drug-resistant markers to study the hybridization of mouse fibroblastsExperimental Cell Research, 1966
- Selection of Hybrids from Matings of Fibroblasts in vitro and Their Presumed RecombinantsScience, 1964
- Deletion of thymidine kinase activity from L cells resistant to bromodeoxyuridineExperimental Cell Research, 1963