The initial stages of adsorption of plasma derived proteins on artificial surfaces in a controlled flow environment
- 1 January 1985
- journal article
- research article
- Published by Wiley in Journal of Biomedical Materials Research
- Vol. 19 (1), 57-66
- https://doi.org/10.1002/jbm.820190106
Abstract
Surfaces of amorphous carbon, polystyrene, and polycarbonate were exposed to solutions of fibrinogen, a modified fibrinogen which lacked the alpha chain protuberance, and serum albumin. The results were studied by electron microscopy. The exposures occurred in a well characterized flow environment at a shear rate of 135/s. All three protein species formed a film when adsorbed to carbon films. When the proteins were adsorbed to polystyrene surfaces, formation of a network was observed. Polycarbonate surfaces adsorbed the proteins both as a network and as a continuous film. It was observed that the nature of the adsorption process depended upon the specific combination of molecule and material. For example, on carbon, individual fibrinogen molecules retain their trinodular structure and adsorb randomly until a monolayer forms. On polystyrene, the individual fibrinogen molecules appear as globules and a network forms before complete coverage occurs.This publication has 18 references indexed in Scilit:
- Protein Adsorption on BiomaterialsPublished by American Chemical Society (ACS) ,1982
- Protein Adsorption on PolymersPublished by American Chemical Society (ACS) ,1982
- Adsorption of fibrinogen on glass: reversibility aspectsJournal of Colloid and Interface Science, 1981
- Dynamics of interactions between human albumin and polyethylene surfaceJournal of Colloid and Interface Science, 1978
- OBSERVATION OF ALBUMIN DEPOSITS ON TEFLON SURFACESASAIO Journal, 1977
- Adsorption of fibrinogen. Electron microscope studyThe Journal of Physical Chemistry, 1971
- Identification of adsorbed protein films by exposure to antisera and water vaporJournal of Biomedical Materials Research, 1969
- Findings with the recording ellipsometer suggesting rapid exchange of specific plasma proteins at liquid/solid interfacesSurface Science, 1969
- Identification of rapid changes at plasma–solid interfacesJournal of Biomedical Materials Research, 1969
- Initial events in interactions of blood with a foreign surfaceJournal of Biomedical Materials Research, 1969