Lineage Commitment and Maturation Induction in Normal and Leukemic Preprogenitor Cells

Abstract

Analysis of the critical cellular processes of self‐generation, commitment, and maturation induction ideally requires the use of clonal cultures using cells with a capacity to undergo all three processes. Preprogenitor cells from normal mouse marrow are proving useful cells for such studies. Cells of a newly established cloned leukemic cell line, the GB2, are providing a useful analogous leukemic system because GB2 cells form stratified subpopulations of clonogenic cells able to be clonally analyzed in vitro in which self‐renewal is demonstrable, but in which near‐normal maturation can be induced by a wide range of hematopoietic regulators.