Purification and viability determinations of plant protoplasts
- 1 January 1976
- journal article
- research article
- Published by Springer Science and Business Media LLC in Planta
- Vol. 128 (3), 213-216
- https://doi.org/10.1007/bf00393231
Abstract
A method is described for purifying plant protoplasts from cellular and subcellular debris. The procedure utilizes a density buffer containing 9.6% sodium metrizoate and 5.6% Ficoll. The use of fluorescein diacetate for assessing the viability of plant protoplasts is also reported.Keywords
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