Displacement chromatography on synthetic ion-exchange resins. 8. A systematic method for the separation of amino-acids

Abstract

A systematic procedure is described for the fractionation and isolation of amino acids and bases using displacement chromatograms carried out with synthetic ion-exchange resins. The procedure is illustrated by 2 model expts. In the 1st example 16 amino acids were isolated from 280 g. of commercial egg albumin with an overall recovery of 54% of the dry weight of protein taken. In the 2d example 10 g. of a protein derived from yeast were hydrolyzed and fractionated. 13 crystalline amino acids were recovered, the total wt. representing 60% of the dry wt. of the protein. A new procedure for the isolation of threonine and serine is described. The relationship between the dissociation constants of a solute and its position in the sequence of displacement is discussed.