Metabolic Profiling: A New Tool in the Study of Wood Formation
- 21 February 2004
- journal article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 52 (6), 1427-1434
- https://doi.org/10.1021/jf034688l
Abstract
In the realm of plant genomics, metabolic profiling has become a valuable tool with which to assess the effect of genetic and/or environmental factors on plant development. This paper reports the first application of metabolic profiling on differentiating xylem tissue of loblolly pine. A protocol is presented for the analysis of loblolly pine xylem tissue. The effects of sample preparation, extraction, and derivatization on the corresponding metabolite profiles and yields have been investigated and are reported. Gas chromatography−mass spectroscopy has been used to quantify >60 polar and lipophilic metabolites from wood-forming tissue. It was possible to assign chemical structures to approximately half of these compounds. Comparison of six loblolly pine genotypes, three high cellulose (50−52%) and three medium (45−48%) cellulose, showed distinct metabolic profiles. Principal component analysis enabled the assignment of metabolic phenotypes using these large data sets. Metabolic phenotype clustering occurred in which the three high-cellulose genotypes were segregated from the medium-cellulose genotypes. These results demonstrate the use of metabolic profiling for the study of wood-forming tissue and as a tool in functional genomics. Keywords: Metabolic profiling; principal component analysis (PCA); loblolly pine; xylem tissue; lipid metabolites; polar metabolitesKeywords
This publication has 10 references indexed in Scilit:
- Metabolic profiling on the right pathNature Biotechnology, 2000
- Metabolite profiling for plant functional genomicsNature Biotechnology, 2000
- Application of high-performance liquid chromatography with photodiode array detection to the metabolic profiling of plant isoprenoidsThe Plant Journal, 2000
- Pushing the accelerator – speeding up drug research with accelerator mass spectrometryNuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 2000
- Identification of Uncommon Plant Metabolites Based on Calculation of Elemental Compositions Using Gas Chromatography and Quadrupole Mass SpectrometryAnalytical Chemistry, 2000
- Metabolic profiling: a rosetta stone for genomics?Current Opinion in Plant Biology, 1999
- A new chemical diagnostic method for inborn errors of metabolism by mass spectrometry—rapid, practical, and simultaneous urinary metabolites analysisMass Spectrometry Reviews, 1996
- Advanced instrumentation and strategies for metabolic profilingJournal of Chromatography B: Biomedical Sciences and Applications, 1986
- Metabolic profiling with gas chromatography—mass spectrometry and its application to clinical medicineJournal of Chromatography B: Biomedical Sciences and Applications, 1986
- Determination of amino acid profiles in biological samples by gas chromatographyJournal of Chromatography A, 1974