K+ secretion in the rat kidney: Na+ channel-dependent and -independent mechanisms
- 1 August 2009
- journal article
- research article
- Published by American Physiological Society in American Journal of Physiology-Renal Physiology
- Vol. 297 (2), F389-F396
- https://doi.org/10.1152/ajprenal.90528.2008
Abstract
Renal Na+ and K+ excretion was measured in rats with varying dietary K+ intake. The requirement for channel-mediated distal nephron Na+ reabsorption was assessed by infusing the animals with the K+-sparing diuretic amiloride via osmotic minipumps. At infusion rates of 2 nmol/min, the concentration of amiloride in the urine was 38 μM, corresponding to concentrations of 9–23 μM in the distal tubular fluid, sufficient to block >98% of Na+ transport through apical Na+ channels (ENaC). With a control K+ intake (0.6% KCl), amiloride reduced K+ excretion rates (UKV) from 0.85 ± 0.15 to 0.05 ± 0.01 μmol/min during the first 2 h of infusion, suggesting that distal nephron K+ secretion was completely dependent on the activity of Na+ channels. When K+ intake was increased by feeding overnight with a diet containing 10% KCl, amiloride reduced UKV from 7.5 ± 0.7 to 1.3 ± 0.1 μmol/min despite an increased plasma K+ of 9 mM, again suggesting a major but not exclusive role for the Na+ channel-dependent pathway of K+ secretion. The maximal measured rates of amiloride-sensitive K+ excretion correspond well with estimates based on apical K+ channel activity in distal nephron segments. However, when the animals were adapted to the high-K+ diet for 7–9 days, the diuretic decreased UKV less, from 6.1 ± 0.6 to 3.0 ± 0.8 μmol/min, indicating an increasing fraction of K+ excretion that was independent of Na+ channels. This indicates the upregulation of a Na+ channel-independent mechanism for secreting K+.Keywords
This publication has 43 references indexed in Scilit:
- Cellular localization of mitochondria contributes to Kv channel-mediated regulation of cellular excitability in pulmonary but not mesenteric circulationAmerican Journal of Physiology-Lung Cellular and Molecular Physiology, 2009
- Dietary K regulates ROMK channels in connecting tubule and cortical collecting duct of rat kidneyAmerican Journal of Physiology-Renal Physiology, 2009
- Basolateral Na+/H+ exchange maintains potassium secretion during diminished sodium transport in the rabbit cortical collecting ductKidney International, 2009
- High-conductance K channels in intercalated cells of the rat distal nephronAmerican Journal of Physiology-Renal Physiology, 2007
- Quantification of K+ secretion through apical low-conductance K channels in the CCDAmerican Journal of Physiology-Renal Physiology, 2005
- Epithelial Na channels and short-term renal response to salt deprivationAmerican Journal of Physiology-Renal Physiology, 2002
- Regulation of Na channels of the rat cortical collecting tubule by aldosterone.The Journal of general physiology, 1993
- Kaliuretic Response to Potassium Loading in Amiloride-Treated DogsKidney and Blood Pressure Research, 1990
- Amiloride and its analogs as tools in the study of ion transportThe Journal of Membrane Biology, 1988
- Electrophysiological properties of cellular and paracellular conductive pathways of the rabbit cortical collecting ductThe Journal of Membrane Biology, 1984