Characterization and Separation of an Inhibitor of Viral Hemagglutination Present in Urine
- 1 May 1950
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 74 (1), 108-114
- https://doi.org/10.3181/00379727-74-17825
Abstract
Normal human urine contains a highly active inhibitor of viral hemagglutination, effective against PR8, Lee, FM1, swine, mumps, Newcastle disease, and GDVII viruses,but inactive against PVM. Urine from cats, rabbits and guinea pigs also shows inhibitory activity. The inhibitor concns. are of the order of 2 mg. %. The inhibitor was isolated from human urine by means of 3 procedures: alcohol precipitation; precipitation at pH 3; and precipitation with 0.58 [image] NaCl. After further purification the material was active at 0.0001 mg./cc. It appears to be a mucoprotein. The activity of the inhibitor as it occurs in urine is inversely related to salt concn.Keywords
This publication has 14 references indexed in Scilit:
- Inhibitory Effect of Cow's Milk on Influenza Virus Hemagglutination.Experimental Biology and Medicine, 1949
- Hemagglutination with the GDVII Strain of Mouse Encephalomyelitis VirusExperimental Biology and Medicine, 1949
- THE NATURE OF THE VIRUS RECEPTORS OF RED CELLSThe Journal of Experimental Medicine, 1949
- THE EGG WHITE INHIBITOR OF INFLUENZA VIRUS HEMAGGLUTINATION .1. PREPARATION AND PROPERTIES OF SEMIPURIFIED INHIBITOR1949
- OVOMUCIN, A SUBSTRATE FOR THE ENZYME OF INFLUENZA VIRUS .1. OVOMUCIN AS AN INHIBITOR OF HAEMAGGLUTINATION BY HEATED LEE VIRUS1949
- STUDIES ON A FACTOR IN NORMAL ALLANTOIC FLUID INHIBITING INFLUENZA VIRUS HAEMAGGLUTINATION - PRECIPITATION-DISSOLUTION REACTION IN MIXTURES OF ACTIVE VIRUS AND INHIBITOR1949
- PURIFICATION OF AN INFLUENZA VIRUS SUBSTRATE, AND DEMONSTRATION OF ITS COMPETITIVE ANTAGONISM TO APPLE PECTINThe Journal of Experimental Medicine, 1949
- The Mechanism of Egg-White Inhibition of Hemagglutination by Swine Influenza Virus.Experimental Biology and Medicine, 1948
- DIMENSIONAL, OSMOTIC, AND CHEMICAL CHANGES OF ERYTHROCYTES IN STORED BLOOD. I. BLOOD PRESERVED IN SODIUM CITRATE, NEUTRAL, AND ACID CITRATE-GLUCOSE (ACD) MIXTURES 1JCI Insight, 1947
- A MODIFIED SALK TEST FOR IN VITRO TITRATION OF INFLUENZA ANTIBODIESImmunology & Cell Biology, 1946