The analysis of blood phospholipins

Abstract

Micro-chemical methods for the detn. of choline and glycerol in lipid solns. are described. Choline is detd. after baryta hydrolysis by precipitation with ammonium reineckate and analysis of the precipitate for N by the micro-Kjeldahl technique. Glycerol is detd. by hydrolysis with caustic soda, removal of glucose, etc., with Cu-lime, and oxidation of the glycerol to formaldehyde with periodic acid. After destruction of the excess periodate, the formaldehyde is distilled off and estimated colorimetrically with phenylhydrazine, K ferricyanide, and mineral acid. These 2 methods have been applied, with a method for lipoid P, to blood. Sphingomyelin appears to account for as much as 56-70% of the phospholipids of whole blood.