FISH localization of human cytoplasmic actin genes ACTB to 7p22 and ACTG1 to 17q25 and characterization of related pseudogenes

Abstract

Human β- and γ-cytoplasmic actin genes (ACTB and ACTG1) were mapped to chromosomes 7p22 and 17q25, respectively, by fluorescence in situ hybridization (FISH). Four processed pseudogenes, β-actin-related ACTBP9 and γ-actin-related ACTGPl, ACTGP3, and ACTGP9, were isolated from human libraries. By PCR of somatic cell hybrid DNAs, ACTBP9 and two β-actin-related pseudogenes (ACTBP7 and ACTBP8) were mapped to human chromosomes 18, 15, and 6, respectively. The γ-actin-related pseudogenes were mapped by FISH to chromosomes 3q23 (ACTGPl), 20pl3 (ACTGP3), and 6p21.1 (ACTGP9).