Streptococcus salivarius Fimbriae Are Composed of a Glycoprotein Containing a Repeated Motif Assembled into a Filamentous Nondissociable Structure
- 1 May 2001
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 183 (9), 2724-32
- https://doi.org/10.1128/jb.183.9.2724-2732.2001
Abstract
Streptococcus salivarius , a gram-positive bacterium found in the human oral cavity, expresses flexible peritrichous fimbriae. In this paper, we report purification and partial characterization of S. salivarius fimbriae. Fimbriae were extracted by shearing the cell surface of hyperfimbriated mutant A37 (a spontaneous mutant of S. salivarius ATCC 25975) with glass beads. Preliminary experiments showed that S. salivarius fimbriae did not dissociate when they were incubated at 100°C in the presence of sodium dodecyl sulfate. This characteristic was used to separate them from other cell surface components by successive gel filtration chromatography procedures. Fimbriae with molecular masses ranging from 20 × 10 6 to 40 × 10 6 Da were purified. Examination of purified fimbriae by electron microscopy revealed the presence of filamentous structures up to 1 μm long and 3 to 4 nm in diameter. Biochemical studies of purified fimbriae and an amino acid sequence analysis of a fimbrial internal peptide revealed that S. salivarius fimbriae were composed of a glycoprotein assembled into a filamentous structure resistant to dissociation. The internal amino acid sequence was composed of a repeated motif of two amino acids alternating with two modified residues: A/X/T-E-Q-M/φ, where X represents a modified amino acid residue and φ represents a blank cycle. Immunolocalization experiments also revealed that the fimbriae were associated with a wheat germ agglutinin-reactive carbohydrate. Immunolabeling experiments with antifimbria polyclonal antibodies showed that antigenically related fimbria-like structures were expressed in two other human oral streptococcal species, Streptococcus mitis and Streptococcus constellatus .Keywords
This publication has 32 references indexed in Scilit:
- Identification of dipeptide repeats and a cell wall sorting signal in the fimbriae‐associated adhesin, Fap1, of Streptococcus parasanguisMolecular Microbiology, 1999
- Taxonomic Note: Necessary Correction of Specific Epithets Formed as Substantives (Nouns) “in Apposition”International Journal of Systematic and Evolutionary Microbiology, 1997
- III. Biochemistry of S-layersFEMS Microbiology Reviews, 1997
- Determination of 16S rRNA Sequences of Streptococcus mitis and Streptococcus gordonii and Phylogenetic Relationships among Members of the Genus StreptococcusInternational Journal of Systematic and Evolutionary Microbiology, 1995
- Non-S-layer glycoproteins in eubacteriaFEMS Microbiology Letters, 1994
- Hydrolytic enzymes and lectin-binding activity of black-pigmented anaerobic rodsMicrobiology, 1994
- Alterations in the cellular envelope of spontaneous IIIManL-defective mutants of Streptococcus salivariusJournal of General Microbiology, 1993
- Control of sugar utilization in oral streptococci. Properties of phenotypically distinct 2‐deoxyglucose‐resistant mutants of Streptococcus salivariusOral Microbiology and Immunology, 1990
- ECOLOGY, PHYSIOLOGY, AND GENETICS OF FIMBRIAE AND PILIAnnual Review of Microbiology, 1975
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970