Identification of a Herpes Simplex Virus Type 1 Polypeptide Which Is a Component of the Virus-induced Ribonucleotide Reductase
- 1 September 1984
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 65 (9), 1457-1466
- https://doi.org/10.1099/0022-1317-65-9-1457
Abstract
Summary We have characterized a temperature-sensitive mutant of herpes simplex virus type 1 (HSV-1), 17tsVP1207, that induces a thermolabile ribonucleotide reductase activity. This mutant was derived from the multiple mutant tsG. Fine-structure mapping studies showed that the defect in 17tsVP1207 lies within an 800 bp sequence between genome map coordinates 0.580 and 0.585 in the gene encoding a polypeptide of 140000 mol. wt. (Vmw136, ICP6). Since the mutation in this polypeptide produced a temperature-sensitive ribonucleotide reductase activity, Vmw136 must be a component of the herpes simplex virus-induced ribonucleotide reductase. The mRNA of Vmw136 has a common 3′ terminus with an mRNA encoding a 38000 mol. wt. polypeptide (Vmw38). Although the polypeptide-coding sequences of these mRNAs do not overlap, monoclonal antibodies against Vmw136 immunoprecipitated Vmw38 as well as Vmw136 from wild-type HSV-1-infected cells. Our data do not exclude the possibility that Vmw38 is part of the ribonucleotide reductase complex but suggest that this species on its own is not responsible for the HSV-induced enzyme activity.Keywords
This publication has 10 references indexed in Scilit:
- Ribonucleotide reductase induced by herpes simplex type 1 virus. Characterization of a distinct enzyme.Journal of Biological Chemistry, 1983
- Ribonucleotide Reductase Induced by Herpes Simplex Virus has a Virus-specified ConstituentJournal of General Virology, 1983
- Induction of a new ribonucleotide reductase after infection of mouse L cells with pseudorabies virusJournal of Virology, 1982
- Fine-structure mapping of herpes simplex virus type 1 temperature-sensitive mutations within the short repeat region of the genomeJournal of Virology, 1981
- Partial purification and characterization of the ribonucleotide reductase induced by herpes simplex virus infection of mammalian cellsJournal of Virology, 1981
- Ribonucleotide reductase from calf thymus. Separation of the enzyme into two nonidentical subunits, proteins M1 and M2.Journal of Biological Chemistry, 1980
- Herpes Simplex Virus Glycoproteins: Isolation of Mutants Resistant to Immune CytolysisJournal of Virology, 1980
- Herpes simplex virus phosphoproteins. I. Phosphate cycles on and off some viral polypeptides and can alter their affinity for DNAJournal of Virology, 1979
- Separation and characterization of herpes simplex virus type 1 immediate-early mRNA'sJournal of Virology, 1979
- Sequence arrangement in herpes simplex virus type 1 DNA: identification of terminal fragments in restriction endonuclease digests and evidence for inversions in redundant and unique sequencesJournal of Virology, 1976