Recovery, Lifespan, and Function of CPD‐Adenine (CPDA‐1) Platelet Concentrates Stored for Up to 72 Hours at 4 C

Abstract

The in vivo recovery, lifespan and hemostatic effectiveness of the anticoagulant-preservative CPDA-1 [citrate-phosphate-dextrose-adenine] platelet concentrates stored for up to 72 h at 4.degree. C were studied. A total of 120 CPDA-1 concentrates containing an average (.+-.1 SD) of 6.6 .+-. 2.0 .times. 1010 platelets were prepared. The pH of the units following storage at 4.degree. C was 6.8 .+-. 0.2; no unit had a pH below 6.3. Autologous transfusion of 6 normal volunteers showed that platelets stored at 4.degree. C for 72 h had an in vivo recovery of 40 .+-. 18% and a lifespan of 5.1 .+-. 1.5 days. The hemostatic effectiveness of CPDA-1 platelets was determined by platelet counts and template bleeding time measurements in 10 thrombocytopenic patients. Patients receiving 48 h stored platelets had a 4-6 h posttransfusion corrected platelet increment averaging 15,300 .+-. 3200/.mu.l which was 67 .+-. 34% of expected recovery. Four of the 5 patients transfused with this preparation showed an improved bleeding time. Three patients receiving 72 h stored platelets had a 4-6 h posttransfusion increment of 5800 .+-. 2400/.mu.l that was only 26 .+-. 13% of the expected recovery. Only 1 of these patients showed any correction of the bleeding time. CPDA-1 platelets are hemostatically effective when stored at 4.degree. C for up to 48 h.