Analysis of adherence, biofilm formation and cytotoxicity suggests a greater virulence potential of Gardnerella vaginalis relative to other bacterial-vaginosis-associated anaerobes
Open Access
- 1 February 2010
- journal article
- Published by Microbiology Society in Microbiology
- Vol. 156 (2), 392-399
- https://doi.org/10.1099/mic.0.034280-0
Abstract
Worldwide, bacterial vaginosis (BV) is the most common vaginal disorder in women of childbearing age. BV is characterized by a dramatic shift in the vaginal microflora, involving a relative decrease in lactobacilli, and a proliferation of anaerobes. In most cases of BV, the predominant bacterial species found is Gardnerella vaginalis. However, pure cultures of G. vaginalis do not always result in BV, and asymptomatic women are sometimes colonized with low numbers of G. vaginalis. Thus, there is controversy about whether G. vaginalis is an opportunistic pathogen and the causative agent of many cases of BV, or whether BV is a polymicrobial condition caused by the collective effects of an altered microbial flora. Recent studies of the biofilm-forming potential and cytotoxic activity of G. vaginalis have renewed interest in the virulence potential of this organism. In an effort to tease apart the aetiology of this disorder, we utilized in vitro assays to compare three virulence properties of G. vaginalis relative to other BV-associated anaerobes. We designed a viable assay to analyse bacterial adherence to vaginal epithelial cells, we compared biofilm-producing capacities, and we assessed cytotoxic activity. Of the BV-associated anaerobes tested, only G. vaginalis demonstrated all three virulence properties combined. This study suggests that G. vaginalis is more virulent than other BV-associated anaerobes, and that many of the bacterial species frequently isolated from BV may be relatively avirulent opportunists that colonize the vagina after G. vaginalis has initiated an infection.Keywords
This publication has 44 references indexed in Scilit:
- Vertebral osteomyelitis and discitis due to Gardnerella vaginalisJournal of Medical Microbiology, 2009
- Aggregatibacter actinomycetemcomitans Builds Mutualistic Biofilm Communities with Fusobacterium nucleatum and Veillonella Species in SalivaInfection and Immunity, 2009
- Gardnerella vaginalis Acute Hip Arthritis in a Renal Transplant RecipientJournal of Clinical Microbiology, 2009
- Polymicrobial Nature of Chronic Diabetic Foot Ulcer Biofilm Infections Determined Using Bacterial Tag Encoded FLX Amplicon Pyrosequencing (bTEFAP)PLOS ONE, 2008
- Diversity of Human Vaginal Bacterial Communities and Associations with Clinically Defined Bacterial VaginosisApplied and Environmental Microbiology, 2008
- Molecular Quantification ofGardnerella vaginalisandAtopobium vaginaeLoads to Predict Bacterial VaginosisClinical Infectious Diseases, 2008
- Functional and Phylogenetic Characterization of Vaginolysin, the Human-Specific Cytolysin fromGardnerella vaginalisJournal of Bacteriology, 2008
- The lower genital tract microbiota in relation to cytokine‐, SLPI‐ and endotoxin levels: application of checkerboard DNA‐DNA hybridization (CDH)APMIS, 2008
- Gardnerella vaginalis Bacteremia in a Previously Healthy Man: Case Report and Characterization of the IsolateJournal of Clinical Microbiology, 2008
- Targeted PCR for Detection of Vaginal Bacteria Associated with Bacterial VaginosisJournal of Clinical Microbiology, 2007