Metabonomic Profiling of Serum and Urine by 1H NMR-Based Spectroscopy Discriminates Patients with Chronic Obstructive Pulmonary Disease and Healthy Individuals
Open Access
- 6 June 2013
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 8 (6), e65675
- https://doi.org/10.1371/journal.pone.0065675
Abstract
Chronic obstructive pulmonary disease (COPD) has seriously impacted the health of individuals and populations. In this study, proton nuclear magnetic resonance ( 1 H NMR)-based metabonomics combined with multivariate pattern recognition analysis was applied to investigate the metabolic signatures of patients with COPD. Serum and urine samples were collected from COPD patients (n = 32) and healthy controls (n = 21), respectively. Samples were analyzed by high resolution 1 H NMR (600 MHz), and the obtained spectral profiles were then subjected to multivariate data analysis. Consistent metabolic differences have been found in serum as well as in urine samples from COPD patients and healthy controls. Compared to healthy controls, COPD patients displayed decreased lipoprotein and amino acids, including branched-chain amino acids (BCAAs), and increased glycerolphosphocholine in serum. Moreover, metabolic differences in urine were more significant than in serum. Decreased urinary 1-methylnicotinamide, creatinine and lactate have been discovered in COPD patients in comparison with healthy controls. Conversely, acetate, ketone bodies, carnosine, m-hydroxyphenylacetate, phenylacetyglycine, pyruvate and α-ketoglutarate exhibited enhanced expression levels in COPD patients relative to healthy subjects. Our results illustrate the potential application of NMR-based metabonomics in early diagnosis and understanding the mechanisms of COPD.Keywords
This publication has 59 references indexed in Scilit:
- Glucose and pyruvate metabolism in severe chronic obstructive pulmonary diseaseJournal of Applied Physiology, 2012
- Targeted metabolomics identifies perturbations in amino acid metabolism that sub-classify patients with COPDMolecular BioSystems, 2012
- T helper type 17-related cytokine expression is increased in the bronchial mucosa of stable chronic obstructive pulmonary disease patientsClinical and Experimental Immunology, 2009
- Amino acids: metabolism, functions, and nutritionAmino Acids, 2009
- 1-Methylnicotinamide and nicotinamide: two related anti-inflammatory agents that differentially affect the functions of activated macrophagesArchivum Immunologiae et Therapiae Experimentalis, 2008
- COPD diagnosis related to different guidelines and spirometry techniquesRespiratory Research, 2007
- Projections of Global Mortality and Burden of Disease from 2002 to 2030PLoS Medicine, 2006
- Definition, epidemiology, and risk factorsBMJ, 2006
- 'Metabonomics': understanding the metabolic responses of living systems to pathophysiological stimuli via multivariate statistical analysis of biological NMR spectroscopic dataXenobiotica, 1999
- Endogenous skeletal muscle antioxidantsCritical Reviews in Food Science and Nutrition, 1994