Nucleolar transcription factor hUBF contains a DNA-binding motif with homology to HMG proteins
- 26 April 1990
- journal article
- Published by Springer Science and Business Media LLC in Nature
- Vol. 344 (6269), 830-836
- https://doi.org/10.1038/344830a0
Abstract
The eukaryotic upstream binding factor (UBF), recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the species-specific factor, SL1. Isolation of complementary DNA clones and sequence analysis reveals similarities between DNA binding domains of human UBF (hUBF) and high mobility group (HMG) protein 1. Expression, cellular localization and in vitro transcription studies establish that cloned hUBF encodes a nucleolar factor that binds specifically to the upstream control element and core of the rRNA gene promoter to activate transcription in a binding site-dependent manner.Keywords
This publication has 39 references indexed in Scilit:
- Transcriptional Regulation in Mammalian Cells by Sequence-Specific DNA Binding ProteinsScience, 1989
- EUKARYOTIC TRANSCRIPTIONAL REGULATORY PROTEINSAnnual Review of Biochemistry, 1989
- Promoter specificity and modulation of RNA polymerase II transcriptionThe FASEB Journal, 1989
- Functional Cooperativity Between Transcription Factors UBF1 and SL1 Mediates Human Ribosomal RNA SynthesisScience, 1988
- Analysis of clustered point mutations in the human ribosomal RNA gene promoter by transient expression in vivo.Proceedings of the National Academy of Sciences of the United States of America, 1988
- TRANSCRIPTION OF CLONED EUKARYOTIC RIBOSOMAL RNA GENESAnnual Review of Biochemistry, 1986
- Human rRNA transcription is modulated by the coordinate binding of two factors to an upstream control elementCell, 1986
- Two distinct promoter elements in the human rRNA gene identified by linker scanning mutagenesis.Molecular and Cellular Biology, 1986
- Purification and characterization of a transcription factor that confers promoter specificity to human RNA polymerase I.Molecular and Cellular Biology, 1985
- Transcription of herpes simplex virus tk sequences under the control of wild-type and mutant human RNA polymerase I promoters.Molecular and Cellular Biology, 1985