Isolation and Functional Analysis of a MYB Transcription Factor Gene that is a Key Regulator for the Development of Red Coloration in Apple Skin
Open Access
- 13 June 2007
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant and Cell Physiology
- Vol. 48 (7), 958-970
- https://doi.org/10.1093/pcp/pcm066
Abstract
Red coloration of apple (Malus × domestica) skin is an important determinant of consumer preference and marketability. Anthocyanins are responsible for this coloration, and their accumulation is positively correlated with the expression level of anthocyanin biosynthetic genes. Regulation of expression of these genes is believed to be controlled by MYB transcription factors, and the MYB transcription factors involved in the activation of anthocyanin biosynthetic genes have been isolated in various plants. In the present study, we isolated and characterized a MYB transcription factor gene (MdMYBA) from apple skin. Characterization of MdMYBA demonstrated that (i) MdMYBA expression was specifically regulated depending on the tissue and cultivar/species; (ii) its expression level was much higher in a deep-red cultivar (‘Jonathan’) than in a pale-red cultivar (‘Tsugaru’); (iii) when cauliflower mosaic virus 35S::MdMYBA was introduced into the cotyledons of apple seedlings by means of a transient assay, reddish-purple spots were induced, and MdMYBA also induced anthocyanin accumulation in reproductive tissues of transgenic tobacco; (iv) the expression of MdMYBA was induced by UV-B irradiation and low-temperature treatment, both of which are known to be important in the promotion of anthocyanin accumulation in apple skin; (v) MdMYBA bound specifically to an anthocyanidin synthase (MdANS) promoter region in a gel-shift assay; and (vi) MdMYBA was mapped to the near region of the BC226-STS (a1) marker for the red skin color locus (Rf). These results suggest that MdMYBA is a key regulatory gene in anthocyanin biosynthesis in apple skin.Keywords
This publication has 38 references indexed in Scilit:
- Suppression subtractive hybridization identifies genes induced in response to UV-B irradiation in apple skin: isolation of a putative UDP-glucose 4-epimeraseJournal of Experimental Botany, 2007
- Red colouration in apple fruit is due to the activity of the MYB transcription factor, MdMYB10The Plant Journal, 2007
- Characterization of a Grapevine R2R3-MYB Transcription Factor That Regulates the Phenylpropanoid PathwayPlant Physiology, 2005
- Activation of Anthocyanin Biosynthesis inGerbera hybrida(Asteraceae) Suggests Conserved Protein-Protein and Protein-Promoter Interactions between the Anciently Diverged Monocots and EudicotsPlant Physiology, 2003
- Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) Function as Transcriptional Activators in Abscisic Acid SignalingPlant Cell, 2002
- Analysis of the genome sequence of the flowering plant Arabidopsis thalianaNature, 2000
- Activation Tagging Identifies a Conserved MYB Regulator of Phenylpropanoid BiosynthesisPlant Cell, 2000
- Expression of anthocyanin biosynthesis pathway genes in red and white grapesPlant Molecular Biology, 1996
- Identification of co-dominant RAPD markers tightly linked to fruit skin color in appleTheoretical and Applied Genetics, 1996
- Postharvest Stimulation of Skin Color in Royal Gala AppleJournal of the American Society for Horticultural Science, 1995