Use of Real-Time PCR To Detect and Quantify Mycoplasma haemofelis and “ Candidatus Mycoplasma haemominutum” DNA

Abstract
A real-time PCR assay using Taqman probes was developed to detect and quantify Mycoplasma haemofelis and “ Candidatus Mycoplasma haemominutum” in feline blood samples. The assay was rapid and sensitive and was successfully used to monitor the in vivo kinetics of cats experimentally infected with each species.

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