Autocatalytic Activation of Trypsinogen in the Presence of Concentrated Ammonium or Magnesium Sulfate

Abstract

By fractional crystallization of an impure crystalline fraction obtained from corpora lutea and known to contain progestin, 4 crystalline compounds were obtained. The main constituent (A) is a physiologically inactive oxy-ketone, C21H34O2, mp. 190[degree]. A 2nd compound (B), crystallizing in prisms, possesses the physiological properties characteristic of the hormone and proves to be a diketone, C21H30O2, mp. 128[degree]. Another compound (C), crystallizing in needles, and melting at 120[degree]-121[degree], is also physiologically potent. The active compounds, B and C, have similar physiological potency, 1 mgm. being a rabbit unit, give identical semicarbazones, and have identical absorption spectra with a high maximum at 240 [mu][mu]. A 4th compound (D), mp. 70[degree]-74[degree], was obtained in insufficient quantity for analysis but proved to be physiologically inactive.