Identification of lactococci and enterococci by colony hybridization with 23S rRNA-targeted oligonucleotide probes
Open Access
- 1 September 1990
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 56 (9), 2927-2929
- https://doi.org/10.1128/aem.56.9.2927-2929.1990
Abstract
Specific sequences of 23S rRNA of Lactococcus lactis, Enterococcus faecalis, Enteroccus faecium, and Enterococcus malodoratus/Enterococcus avium were identified, and complementary oligonucleotide probes were synthesized. The specificity of the probes was evaluated by dot blot and colony hybridizations. The probes can be used for the specific detection and identification of colonies of the corresponding species in mixed cultures.This publication has 11 references indexed in Scilit:
- The 23S ribosomal RNA higher‐order structure of Pseudomonas cepacia and other prokaryotesEuropean Journal of Biochemistry, 1989
- A rapid procedure for colony screening using nylon filtersNucleic Acids Research, 1989
- DNA Probes with Different Specificities from a Cloned 23S rRNA Gene of Micrococcus luteusMicrobiology, 1988
- Reverse Transcriptase Sequencing of 16S Ribosomal RNA from Faenia rectivirgula, Pseudonocardia thermophila and Saccharopolyspora hirsuta, Three Wall Type IV Actinomycetes Which Lack Mycolic AcidsMicrobiology, 1988
- Phylogenetic group-specific oligodeoxynucleotide probes for identification of single microbial cellsJournal of Bacteriology, 1988
- Analytical strategies for the use of DNA probesAnalytical Biochemistry, 1988
- Detection of hemolytic Listeria monocytogenes by using DNA colony hybridizationApplied and Environmental Microbiology, 1987
- DNA hybridization probe for the Pseudomonas fluorescens groupApplied and Environmental Microbiology, 1986
- Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.Proceedings of the National Academy of Sciences, 1975
- A membrane-filter technique for the detection of complementary DNABiochemical and Biophysical Research Communications, 1966