Diospyrin, A Bisnaphthoquinone: A Novel Inhibitor of Type I DNA Topoisomerase ofLeishmania donovani
- 1 December 1998
- journal article
- Published by American Society for Pharmacology & Experimental Therapeutics (ASPET)
- Vol. 54 (6), 994-999
- https://doi.org/10.1124/mol.54.6.994
Abstract
Diospyrin is a plant product that has significant inhibitory effect on the growth of Leishmania donovani promastigotes. This compound inhibits the catalytic activity of DNA topoisomerase I of the parasite. Like camptothecin, it induces topoisomerase I mediated DNA cleavage in vitro. Treatment of DNA with diospyrin before addition of topoisomerase I has no effect. Preincubation of topoisomerase I with diospyrin before the addition of DNA in the relaxation reaction increases this inhibition. Our results suggest that this bis-naphthoquinone compound exerts its inhibitory effect by binding with the enzyme and stabilizing the topoisomerase I-DNA “cleavable complex.” Diospyrin is a specific inhibitor of the parasitic topoisomerase I. It does not inhibit type II topoisomerase ofL. donovani and requires much higher concentrations to inhibit type I topoisomerase of calf thymus. The potent inhibitory effect of diospyrin on type I DNA topoisomerase fromL. donovani can be exploited for rational drug design in human leishmaniasis.Keywords
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