Extracellularly truncated desmoglein 1 compromises desmosomes in MDCK cells
- 1 January 2000
- journal article
- research article
- Published by Informa UK Limited in Molecular Membrane Biology
- Vol. 17 (3), 175-183
- https://doi.org/10.1080/09687680010002238
Abstract
The formation and stability of epithelial tissue involves cell adhesion and the connection of the intermediate filaments of contiguous cells, mediated by desmosomes. The cadherin family members Desmocollins (Dsc) and Desmogleins (Dsg) mediate desmosome extracellular adhesion. The main intracellular molecules identified linking Dscs and Dsgs with the intermediate filament network are Plakoglobin (PG), Plakophilins (PPs) and Desmoplakin (DP). Previous studies on desmosome-mediated adhesion have focused on the intracellular domains of Dsc and Dsg because of their capacity to interact with PG, PPs and DP. This study examines the role of the extracellular domain of Dsg1 upon desmosome stability in MDCK cells. Dsg1 was constructed containing an extracellular deletion (Dsg delta 1EC) and was expressed in MDCK cells. A high expressor Dsg delta 1EC/MDCK clone was obtained and analysed for its capacity to form desmosomes in cell monolayers and when growing under mechanical stress in three-dimensional collagen cultures. Phenotypic changes associated with the ectopic expression of Dsg1 delta EC in MDCK cells were: disturbance of the cytokeratin network, a change in the quality and number of desmosomes and impairment of the formation of cysts in suspension cultures. Interestingly, Dsg1 delta EC was not localized in desmosomes, but was still able to maintain its intracytoplasmic interaction with PG, suggesting that the disruptive effects were largely due to PG and/or PP sequestration.Keywords
This publication has 38 references indexed in Scilit:
- The Function of Plakophilin 1 in Desmosome Assembly and Actin Filament OrganizationThe Journal of cell biology, 2000
- Clustered Cadherin Genes: A Sequence-Ready Contig for the Desmosomal Cadherin Locus on Human Chromosome 18Genomics, 1999
- Antisense expression of a desmocollin gene in MDCK cells alters desmosome plaque assembly but does not affect desmoglein expressionEuropean Journal of Cell Biology, 1998
- Hierarchical expression of desmosomal cadherins during stratified epithelial morphogenesis in the mouseDifferentiation, 1997
- Analysis of Desmosomal Cadherin–Adhesive Function and Stoichiometry of Desmosomal Cadherin-Plakoglobin ComplexesJournal of Investigative Dermatology, 1996
- Desmosomal Cadherin Binding Domains of PlakoglobinPublished by Elsevier BV ,1996
- Delayed Assembly of Desmosomes in Keratinocytes with Disrupted Classic-Cadherin-Mediated Cell Adhesion by a Dominant Negative MutantJournal of Investigative Dermatology, 1995
- Identification of the plakoglobin-binding domain in desmoglein and its role in plaque assembly and intermediate filament anchorage.The Journal of cell biology, 1994
- Desmosomes and Hemidesmosomes: Constitutive Molecular ComponentsAnnual Review of Cell Biology, 1990
- The assembly of the major desmosome glycoproteins of Madin‐Darby canine kidney cellsFEBS Letters, 1989