Morphogenesis in the yeast cell cycle: regulation by Cdc28 and cyclins.
Open Access
- 15 March 1993
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 120 (6), 1305-1320
- https://doi.org/10.1083/jcb.120.6.1305
Abstract
Analysis of cell cycle regulation in the budding yeast Saccharomyces cerevisiae has shown that a central regulatory protein kinase, Cdc28, undergoes changes in activity through the cell cycle by associating with distinct groups of cyclins that accumulate at different times. The various cyclin/Cdc28 complexes control different aspects of cell cycle progression, including the commitment step known as START and mitosis. We found that altering the activity of Cdc28 had profound effects on morphogenesis during the yeast cell cycle. Our results suggest that activation of Cdc28 by G1 cyclins (Cln1, Cln2, or Cln3) in unbudded G1 cells triggers polarization of the cortical actin cytoskeleton to a specialized pre-bud site at one end of the cell, while activation of Cdc28 by mitotic cyclins (Clb1 or Clb2) in budded G2 cells causes depolarization of the cortical actin cytoskeleton and secretory apparatus. Inactivation of Cdc28 following cyclin destruction in mitosis triggers redistribution of cortical actin structures to the neck region for cytokinesis. In the case of pre-bud site assembly following START, we found that the actin rearrangement could be triggered by Cln/Cdc28 activation in the absence of de novo protein synthesis, suggesting that the kinase may directly phosphorylate substrates (such as actin-binding proteins) that regulate actin distribution in cells.Keywords
This publication has 60 references indexed in Scilit:
- Direct induction of G1-specific transcripts following reactivation of the Cdc28 kinase in the absence of de novo protein synthesis.Genes & Development, 1992
- A proliferation of cyclinsTrends in Cell Biology, 1992
- Population explosion in the cyclin familyCurrent Biology, 1991
- Universal control mechanism regulating onset of M-phaseNature, 1990
- Effects of cytochalasin and phalloidin on actin.The Journal of cell biology, 1987
- The Involvement of Cell Wall Expansion in the Two Modes of Mycelium Formation of Candida albicansMicrobiology, 1985
- Temporal and Spatial Differences in Cell Wall Expansion during Bud and Mycelium Formation in Candida albicansMicrobiology, 1985
- Structural rearrangements of tubulin and actin during the cell cycle of the yeast Saccharomyces.The Journal of cell biology, 1984
- Wall Replication in Saccharomyces Species: Use of Fluorescein-conjugated Concanavalin A to Reveal the Site of Mannan InsertionJournal of General Microbiology, 1972
- Some Aspects of Cell Division in Saccharomyces cerevisiaeJournal of General Microbiology, 1950