A multipurpose immobilized biocatalyst with pectinase, xylanase and cellulase activities
Open Access
- 8 June 2007
- journal article
- Published by Springer Science and Business Media LLC in Chemistry Central Journal
- Vol. 1 (1), 16
- https://doi.org/10.1186/1752-153x-1-16
Abstract
The use of immobilized enzymes for catalyzing various biotransformations is now a widely used approach. In recent years, cross-linked enzyme aggregates (CLEAs) have emerged as a novel and versatile biocatalyst design. The present work deals with the preparation of a CLEA from a commercial preparation, Pectinex™ Ultra SP-L, which contains pectinase, xylanase and cellulase activities. The CLEA obtained could be used for any of the enzyme activities. The CLEA was characterized in terms of kinetic parameters, thermal stability and reusability in the context of all the three enzyme activities.Keywords
This publication has 25 references indexed in Scilit:
- Hydrolysis of apple pectin by the coordinated activity of pectic enzymesFood Chemistry, 2007
- New enzymes for biotransformationsCurrent Opinion in Chemical Biology, 2005
- Cross-linked enzyme aggregates (CLEAs): A novel and versatile method for enzyme immobilization (a review)Biocatalysis and Biotransformation, 2005
- Extraction of oil from Jatropha curcas L. seed kernels by combination of ultrasonication and aqueous enzymatic oil extractionBioresource Technology, 2004
- Solubilization of onion with polysaccharide-degrading enzymesInternational Journal of Food Science & Technology, 2002
- Biotechnology of Microbial Xylanases: Enzymology, Molecular Biology, and ApplicationCritical Reviews in Biotechnology, 2002
- Cross-Linked Enzyme Crystals (CLECs) of Thermolysin in the Synthesis of PeptidesJournal of the American Chemical Society, 1995
- Measurement of cellulase activitiesPure and Applied Chemistry, 1987
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Use of Dinitrosalicylic Acid Reagent for Determination of Reducing SugarAnalytical Chemistry, 1959