Characteristics and Dynamics of Salmonella Contamination along the Coast of Agadir, Morocco
- 15 December 2009
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 75 (24), 7700-7709
- https://doi.org/10.1128/aem.01852-09
Abstract
The occurrence of Salmonella enterica in the environment of tropical and desert regions has remained largely uninvestigated in many areas of the world, including Africa. In the present study, we investigated the presence of Salmonella spp. along 122 km of the coastline of Agadir (southern Morocco) in relation to environmental parameters. A total of 801 samples of seawater (243), marine sediment (279), and mussels (279) were collected from six sites between July 2004 and May 2008. The overall prevalence of Salmonella spp. was 7.1%, with the highest occurrence in mussels (10%), followed by sediment (6.8%) and seawater (4.1%). Only three serotypes were identified among the 57 Salmonella sp. strains isolated. S. enterica serotype Blockley represented 43.8% of all Salmonella strains and was identified in mussel and sediment samples. S. enterica serotype Kentucky (29.8%) was found almost exclusively in mussels, whereas S. enterica serotype Senftenberg (26.3%) was detected in sediment and seawater. Statistical analysis using generalized additive models identified seawater temperature, environmental temperature, rainfall, and solar radiation as significant factors associated with the presence of Salmonella . Rainfall was the only variable showing a linear positive effect on the presence of Salmonella in the sea, whereas the remaining variables showed more complex nonlinear effects. Twenty-eight (49.1%) Salmonella isolates displayed resistance to ampicillin (22 isolates), nalidixic acid (9 isolates), sulfonamide compounds (2 isolates), and tetracycline (1 isolate), with six of these isolates displaying multiple resistance to two of these antimicrobial agents. Pulsed-field gel electrophoresis analysis revealed homogenous restriction patterns within each serotype that were uncorrelated with the resistance pattern profiles.Keywords
This publication has 30 references indexed in Scilit:
- Distribution, Diversity, and Seasonality of Waterborne Salmonellae in a Rural WatershedApplied and Environmental Microbiology, 2009
- Molecular Phylogeny of the Salmonellae: Relationships among Salmonella Species and Subspecies Determined from Four Housekeeping Genes and Evidence of Lateral Gene Transfer EventsJournal of Bacteriology, 2008
- Climate Patterns Governing the Presence and Permanence of Salmonellae in Coastal Areas of Bahia de Todos Santos, MexicoApplied and Environmental Microbiology, 2008
- Antimicrobial resistance of Salmonella isolated from poultry carcasses in Dakar (Senegal)Brazilian Journal of Microbiology, 2006
- Prevalence of Salmonella spp. in Oysters in the United StatesApplied and Environmental Microbiology, 2005
- Stable and Efficient Multiple Smoothing Parameter Estimation for Generalized Additive ModelsJournal of the American Statistical Association, 2004
- FoodNet Estimate of the Burden of Illness Caused by NontyphoidalSalmonellaInfections in the United StatesClinical Infectious Diseases, 2004
- Influence of Environmental Factors and Human Activity on the Presence of Salmonella Serovars in a Marine EnvironmentApplied and Environmental Microbiology, 2004
- Prevalence ofSalmonellain fish and crustaceans from markets in Coimbatore, South IndiaFood Microbiology, 1997
- Influence du passage de Salmonella spp. et E. coli en eaux usees sur leur survie ulterieure en eau de merWater Research, 1995