Human mast cells express androgen receptors but treatment with testosterone exerts no influence on IgE‐independent mast cell degranulation elicited by neuromuscular blocking agents
- 11 February 2010
- journal article
- Published by Wiley in Experimental Dermatology
- Vol. 19 (3), 302-304
- https://doi.org/10.1111/j.1600-0625.2009.00969.x
Abstract
Women predominate in the anaphylactic reactions to neuromuscular blocking agents (NMBA). The expression of oestrogen receptors has been demonstrated in mast cells and oestrogen treatment can enhance mast cell degranulation, but the influence of androgens remains largely unclear. Our immunocytochemical study showed the expression of androgen receptor (AR) in mast cells isolated from human foreskin as well as in two human mast cell lines, HMC-1 and LAD2. The amount of AR was most abundant in human skin mast cells as determined by real-time polymerase chain reaction analysis. Treatment of the HMC-1 mast cells with testosterone or 17beta-oestradiol, alone or in combination with different NMBA, did not affect mast cell degranulation as measured by the release of beta-hexosaminidase. Our study shows for the first time the expression of AR in human skin mast cells. Further studies using primary human mast cell cultures are needed to understand whether and how sex hormones can influence mast cell activation.Keywords
This publication has 19 references indexed in Scilit:
- Testosterone Reduces Macrophage Expression in the Mouse of Toll-Like Receptor 4, a Trigger for Inflammation and Innate ImmunityBiology of Reproduction, 2008
- Inhibition of the Androgen Receptor by Antisense Oligonucleotides Regulates the Biological Activity of Androgens in SZ95 SebocytesHormone and Metabolic Research, 2007
- Estradiol activates mast cells via a non-genomic estrogen receptor-α and calcium influxMolecular Immunology, 2006
- The Risk of Anaphylactic Reactions to Rocuronium in the United States Is Comparable to That of Vecuronium: An Analysis of Food and Drug Administration Reporting of Adverse EventsAnesthesia & Analgesia, 2005
- Anaphylaxis during Anesthesia in NorwayAnesthesiology, 2005
- Anaphylaxis during anesthesia: results of a 12‐year survey at a French pediatric centerAllergy, 2005
- Expression and localization of steroid receptors in human nasal mucosaActa Oto-Laryngologica, 2004
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001
- Androgen Formation and Metabolism in the Pulmonary Epithelial Cell Line A549: Expression of 17 -Hydroxysteroid Dehydrogenase Type 5 and 3 -Hydroxysteroid Dehydrogenase Type 3Endocrinology, 2000
- Heterogeneity of Human Mast Cells and Basophils in Response to Muscle RelaxantsAnesthesiology, 1991