B-lymphocyte heterogeneity: development and characterization of an alloantiserum which distinguishes B-lymphocyte differentiation alloantigens.
Open Access
- 1 January 1977
- journal article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 145 (1), 101-110
- https://doi.org/10.1084/jem.145.1.101
Abstract
CBA/N mice and F1 male mice, which are hemizygous for the CBA/N X chromosome, have an immune defect which is associated with the absence (deficiency) of a subpopulation of mature or late developing B lymphocytes. This characteristic was utilized to develop an antiserum that was specific for this subclass of B cells. C57BL/L mice were immunized with DBA/2 spleen calls, and the resulting antisera was absorbed with lymphoid cells derived from immunologically abnormal (CBA/N female X DBA/2 male)F1 male mice. The absorbed antisera was cytotoxic for a subpopulation of lymphocytes that was present in the spleens of adult DBA/2 and (CBA/N female X DBA/2 male)F1 female mice. The cells killed by the absorbed antisera were Ig-bearing, complement receptor-bearing B lymphocytes, which had a low-to-intermediate density of total surface Ig. Moreover, the cells remaining after treatment of adult (CBA/N female X DBA/2 male)F1 female spleen cells with the absorbed antisera and C had a high ratio of surface IgM to IgD. The development of this cytotoxic alloantisera, which is specific for a late developing (mature) subpopulation of B lymphocytes, will allow the functional characterization of subclasses of B lymphocytes.Keywords
This publication has 12 references indexed in Scilit:
- X-linked B-lymphocyte defect in CBA/N mice. III. Abnormal development of B-lymphocyte populations defined by their density of surface immunoglobulin.The Journal of Experimental Medicine, 1976
- Rapid Isolation of Antigens from Cells with A Staphylococcal Protein A-Antibody Adsorbent: Parameters of the Interaction of Antibody-Antigen Complexes with Protein AJournal Of Immunology, 1975
- X-linked B-lymphocyte immune defect in CBA/N mice. II. Studies of the mechanisms underlying the immune defect.The Journal of Experimental Medicine, 1975
- Functional Analysis of Murine and Human B Lymphocyte SubsetsImmunological Reviews, 1975
- X-linked B-lymphocyte immune defect in CBA/HN mice. I. Studies of the function and composition of spleen cells.The Journal of Experimental Medicine, 1975
- Cell surface immunoglobulin. XI. The appearance of an IgD-like molecule on murine lymphoid cells during ontogeny.The Journal of Experimental Medicine, 1975
- ONTOGENY OF B LYMPHOCYTESThe Journal of Experimental Medicine, 1974
- The Genetics of the Immune Response to a Synthetic Double-Stranded RNA in a Mutant CBA Mouse StrainThe Journal of Immunology, 1973
- GENETIC CONTROL OF THE ANTIBODY RESPONSE TO TYPE III PNEUMOCOCCAL POLYSACCHARIDE IN MICEThe Journal of Experimental Medicine, 1972
- The immunologic response to xenografts. Recognition of mouse H-2 histocompatibility antigens by the rat.1971