THE ASSAY OF ANTIDIURETIC MATERIAL IN BLOOD AND URINE

Abstract

Antidiuretic material in tissues, blood, and urine may be estimated when a dog with exptl. diabetes insipidus is the test animal, and creatinine U/P ratios are followed after the intravenous adm. of the unknown material. Large vols. of untreated urine or dog''s blood may be injected. The response to the unknown is compared to the responses to 2 standard doses of pituitrin, one preceding and one following. Duplicate assays should agree to within 0.3 M.U. to be acceptable. The useful range of dosage is 0.3-3.0 M.U. of pituitrin, when the basal urine flow is more than 1.5; at lower urine flows the threshold and maximal doses are lower.