Characterization of neural cell adhesion molecules (NCAM) expressed by ewing and neuroblastoma cell lines
- 15 July 1987
- journal article
- research article
- Published by Wiley in International Journal of Cancer
- Vol. 40 (1), 81-86
- https://doi.org/10.1002/ijc.2910400115
Abstract
The status of the neural cell adhesion molecule NCAM gene which is mapped to human chromosome 11q23–24 has been investigated in Ewing-tumor-derived cell lines which present the t(11;22)(q23–24;q12) translocation characteristic of this malignancy. No rearrangement was detected when 2 different non-overlapping probes to mouse NCAM were used. The expression of the NCAM gene was analysed at both the protein and messenger levels in material extracted from Ewing cell lines, human neuroblastoma cell line and fetal mouse brain. Immune blot and immunoprecipitation studies showed that the neuroblastoma cell line contained more NCAM material than the Ewing lines. In neuroblastoma but not in Ewing, the NCAM material had the electrophoretic characteristics of molecules with long polysialic acid chains. After treatment with endosialidase, the diffusely migrating neuroblastoma material was resolved into 3 discrete bands of 120, 140 and 180 kDa. In Ewing extract, high-molecular-weight NCAM species were also detected with a 3-band pattern more reminiscent of mature brain. Endoglycosidase F treatment of Ewing NCAM indicated that all 3 species were largely N- glycosylated. Northern blot analysis confirmed that NCAM was expressed more abundantly in neuroblastoma than in Ewing cell lines. Among the 4 NCAM messengers (7.0, 6.5, 4.3 and 4.1 kb) detected in the neuroblastoma, the 6.5 kb species was largely predominant. The Ewing messenger RNA pattern was clearly different as the largest 7.0-kb species was virtually absent and the other bands were of similar intensities.Keywords
This publication has 24 references indexed in Scilit:
- Differential expression of mouse neural cell-adhesion molecule (N-CAM) mRNA species during brain development and in neural cell linesJournal of Neuroscience, 1986
- CELL ADHESION AND THE MOLECULAR PROCESSES OF MORPHOGENESISAnnual Review of Biochemistry, 1985
- Cell‐Free Synthesis of the D2‐Cell Adhesion Molecule: Evidence for Three Primary Translation ProductsJournal of Neurochemistry, 1985
- Mapping of three carbohydrate attachment sites in embryonic and adult forms of the neural cell adhesion molecule.The Journal of cell biology, 1984
- Alterations in neural cell adhesion molecules during development of different regions of the nervous systemJournal of Neuroscience, 1984
- Studies on the transmembrane disposition of the neural cell adhesion molecule N‐CAMEuropean Journal of Biochemistry, 1984
- A technique for radiolabeling DNA restriction endonuclease fragments to high specific activityAnalytical Biochemistry, 1984
- Quantification of the D2‐Glycoprotein in Amniotic Fluid and Serum from Pregnancies with Fetal Neural Tube DefectsJournal of Neurochemistry, 1983
- RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexaminationBiochemistry, 1977
- Ultrastructure of Spontaneously Differentiated Human Malignant Melanocytes Cultured From Primary Tumors2JNCI Journal of the National Cancer Institute, 1977