Selective Determination of Epinephrine in the Presence of Ascorbic Acid and Uric Acid by Electrocatalytic Oxidation at Poly(eriochrome Black T) Film-modified Glassy Carbon Electrode
Open Access
- 10 June 2007
- journal article
- Published by Springer Science and Business Media LLC in Analytical Sciences
- Vol. 23 (6), 677-682
- https://doi.org/10.2116/analsci.23.677
Abstract
An electropolymerized film of eriochrome black T (EBT) has been prepared at a glassy carbon electrode (GCE) by cyclic voltammetry (CV). The poly(EBT) membrane at GCE exhibits an excellent electrocatalytic activity towards the oxidation of epinephrine (EP), ascorbic acid (AA) and uric acid (UA) in acidic solution and reduced the overpotential for the oxidation of EP. The poly(EBT)-coated electrode could separately detect EP, AA and UA in their mixture with the potential differences of 180 and 160 mV for EP-AA and UA-EP, respectively, which are large enough to allow for determination of EP in the presence of AA and UA. Using differential pulse voltammetry, the peak current of EP recorded in pH 3.5 solution was linearly dependent on EP's concentration in the range of 2.5 - 50 µM. Due to its good selectivity and stability, the polymer-coated GCE was successfully applied to the determination of EP in real samples.Keywords
This publication has 21 references indexed in Scilit:
- Flow‐injection chemiluminescence determination of catecholamines based on their enhancing effects on the luminol–potassium periodate systemLuminescence, 2006
- Anodic square-wave stripping voltammetric analysis of epinephrine using carbon fiber microelectrodeMicrochemical Journal, 2006
- Electrochemical self-assembly of nordihydroguaiaretic acid/Nafion modified electrodes and their electrocatalytic properties for catecholamines and ascorbic acidJournal of Electroanalytical Chemistry, 2005
- A glassy carbon supported bilayer lipid-like membrane of 5,5-ditetradecyl-2-(2-trimethyl-ammonioethyl)-1,3-dioxane bromide for electrochemical sensing of epinephrineElectrochimica Acta, 2004
- Effect of Ionic Liquids as Mobile Phase Additives on Retention of Catecholamines in Reversed-Phase High-Performance Liquid ChromatographyAnalytical Letters, 2003
- Catecholamines derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole: characterization of chemical structure and fluorescence propertiesAnalytica Chimica Acta, 2002
- Electrochemical behavior of epinephrine at ?-cysteine self-assembled monolayers modified gold electrodeTalanta, 2002
- Novel and sensitive high-performance liquid chromatographic method based on electrochemical coulometric array detection for simultaneous determination of catecholamines, kynurenine and indole derivatives of tryptophanJournal of Chromatography B, 2002
- A sensitive and selective spectrophotometric estimation of catechol derivatives in pharmaceutical preparationsTalanta, 2001
- Amperometric determination of epinephrine with an osmium complex and Nafion double-layer membrane modified electrodeAnalytica Chimica Acta, 1999