ISOLATION AND FRACTIONATION OF RAT BRAIN NUCLEI
Open Access
- 1 August 1966
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 30 (2), 405-415
- https://doi.org/10.1083/jcb.30.2.405
Abstract
A method for isolating pure and unaltered nuclei from rat brain by means of differential centrifugation is described. The isolated nuclei are further separated into discrete fractions of neuronal, astrocytic, and glial nuclei, with a yield amounting to 20 to 25% of the DNA of the original homogenate. Both the morphology and size of the nuclei remained unchanged. Problems concerning the composition of the isolation media, the use of detergents, as well as those raised by density gradient centrifugation in sucrose, Ficoll, and Dextran are discussed. Some values for the density of each type of brain nuclei are suggested.Keywords
This publication has 14 references indexed in Scilit:
- ISOLATION AND SOME CHARACTERISTICS OF CELL NUCLEI FROM BRAIN CORTEX OF ADULT CATThe Journal of cell biology, 1965
- ISOLATION OF NUCLEI FROM MAMMALIAN TISSUES THROUGH THE USE OF TRITON X-100Journal of Histochemistry & Cytochemistry, 1964
- Biochemistry of the developing rat brain I. Soluble enzymes in isolated neonatal brain nucleiBiochimica et Biophysica Acta, 1963
- A density gradient for the isolation of metabolically active thymus nucleiBiochimica et Biophysica Acta, 1963
- THE ISOLATION OF CELL NUCLEI FROM RAT BRAINThe Journal of cell biology, 1962
- Fractionation of rat liver nuclei into diploid and tetraploid DNA classes by continuous density gradient sedimentationExperimental Cell Research, 1962
- Possible Use of Synthetic Surface-active Agents for the Preparation of Tumour Cell NucleiNature, 1958
- Isolation of pure and unaltered liver nuclei morphology and biochemical compositionExperimental Cell Research, 1956
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951
- Isolated nuclei from cells of the cerebral cortex. Preparation and enzyme contentBiochemical Journal, 1951