Protective action of aqueous black tea (Camellia sinensis) extract (BTE) against ovariectomy‐induced oxidative stress of mononuclear cells and its associated progression of bone loss
- 10 March 2009
- journal article
- research article
- Published by Wiley in Phytotherapy Research
- Vol. 23 (9), 1287-1294
- https://doi.org/10.1002/ptr.2719
Abstract
The protective action of aqueous black tea extract (BTE) against ovariectomy-induced oxidative stress of mononuclear cells and its associated progression of bone loss was demonstrated in this study. Eighteen female adult 6-month-old Wistar albino rats were divided into three groups: sham-control (A), bilaterally ovariectomized (B) and bilaterally ovariectomized + BTE supplemented (C). Studies included the measurement of oxidative (nitric oxide, lipid peroxidation) and antioxidative (superoxide dismutase, catalase) markers, inflammatory cytokines (IL-6, TNF-α), osteoclast differentiation factor (RANKL) and bone resorption markers (tartrate-resistant acid phosphatase and hydroxyproline). Also quantitative histomorphometry and histological studies were undertaken. The bone breaking force was measured. The results indicate that BTE was effective in preserving and restoring skeletal health by reducing the number of active osteoclasts. Such changes with BTE supplementation were steadily linked with the reduced oxidative stress of mononuclear cells, serum levels of bone resorbing cytokines, osteoclast differentiation factor and resorption markers. The results of the bone breaking force, histological and histomorphometric analyses further supported the hypothesis. This study suggests that BTE has both protective and restorative actions against ovariectomy-induced mononuclear cell oxidative stress and associated bone loss. Copyright © 2009 John Wiley & Sons, Ltd.Keywords
This publication has 56 references indexed in Scilit:
- B and T Lymphocytes Are the Primary Sources of RANKL in the Bone Resorptive Lesion of Periodontal DiseaseThe American Journal of Pathology, 2006
- Protection from experimental colitis by theaflavin‐3,3′‐digallate correlates with inhibition of IKK and NF‐κB activationBritish Journal of Pharmacology, 2006
- Phytoestrogenic effects of black tea extract (Camellia sinensis) in an oophorectomized rat (Rattus norvegicus) model of osteoporosisLife Sciences, 2005
- Regulatory Mechanisms Operative in OsteoclastsCritical Reviews™ in Eukaryotic Gene Expression, 2004
- Association between Oxidative Stress and Bone Mineral DensityBiochemical and Biophysical Research Communications, 2001
- Black Tea Is a Powerful Chemopreventor of Reactive Oxygen and Nitrogen Species: Comparison with Its Individual Catechin Constituents and Green TeaBiochemical and Biophysical Research Communications, 2001
- THE NF-κB AND IκB PROTEINS: New Discoveries and InsightsAnnual Review of Immunology, 1996
- High-Lipid Diet Intake Is a Possible Predisposing Factor in the Development of Hypogonadal Osteoporosis.The Japanese Journal of Physiology, 1996
- Estrogen deficiency stimulates B lymphopoiesis in mouse bone marrow.JCI Insight, 1994
- Bone histomorphometry: Standardization of nomenclature, symbols, and units: Report of the asbmr histomorphometry nomenclature committeeJournal of Bone and Mineral Research, 1987