Iron Deficiency Alters Discrete Proteins in Rat Caudate Nucleus and Nucleus Accumbens
- 1 September 1986
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 47 (3), 794-799
- https://doi.org/10.1111/j.1471-4159.1986.tb00681.x
Abstract
Young rats (21 days old) made nutritionally iron deficient, by feeding them a semisynthetic diet containing skimmed milk for 5 weeks, had significantly lowered hemoglobin levels (5.2 ± 4 g/100 ml). The nonheme iron content in caudate nucleus was decreased by 47%. The behavioral response of iron-deficient rats to apomorphine (2 mg/kg) and the density of 3,4-dihydroxyphenylethylamine (dopamine) D2 receptors, as measured by [3H]spiperone binding in caudate nucleus, were significantly reduced by 70 and 53%, respectively. The possibility that nutritional iron deficiency may affect protein content in brain was investigated by measuring the apparent concentration of proteins in caudate nucleus and nucleus accumbens from iron-deficient and control animals using two-dimensional gel electrophoresis. The data indicate that iron deficiency can affect content in these two brain regions. Significant changes in the content of 10 proteins were noted in the caudate nucleus and nucleus accumbens in iron-deficient rats. The albumin level was significantly increased in both regions studied, whereas the neuron-specific enolase level was increased in the nucleus accumbens and the glial fibrillary acidic protein level was reduced in the caudate nucleus. The significance of these protein content changes, as well as a reduction in content of a 94-kilodalton protein (a molecular size similar to that of the D2 dopamine receptor), remains to be established.Keywords
This publication has 28 references indexed in Scilit:
- Effect of Iron Chelators on Dopamine D2 ReceptorsJournal of Neurochemistry, 1985
- Developmental deficits in iron-deficient infants: Effects of age and severity of iron lackThe Journal of Pediatrics, 1982
- Nutritional iron and dopamine binding sites in the rat brainPharmacology Biochemistry and Behavior, 1982
- Long-term effects of early iron deficiency on consummatory behavior in the ratPharmacology Biochemistry and Behavior, 1981
- The effects of iron deficiency on brain biogenic monoamine biochemistry and function in ratsNeuropharmacology, 1980
- Long-term consequences of early iron deficiency in the ratPharmacology Biochemistry and Behavior, 1979
- BIOCHEMICAL AND BEHAVIOURAL ASPECTS OF SIDEROPENIABritish Journal of Haematology, 1979
- The effects of therapy on the developmental scores of iron-deficient infantsThe Journal of Pediatrics, 1978
- Biogenic Monoamine Metabolism and Functional Activity in Iron‐Deficient Rats: Behavioural CorrelatesPublished by Wiley ,1977
- Iron deficiency and behaviorThe Journal of Pediatrics, 1976