An ultrastructural study on the ear cartilage of rabbits after the administration of papain
- 1 February 1981
- journal article
- research article
- Published by Springer Science and Business Media LLC in Virchows Archiv
- Vol. 390 (2), 139-150
- https://doi.org/10.1007/bf02215980
Abstract
Crude papain was administered intravenously to young rabbits and the cartilage of the collapsed ear was examined electron-microscopically. Degeneration and recovery of chondrocytes, and decrease in and recovery of the electron-density of elastic fibers, were observed during the collapse and restoration of the ear. Some samples were stained with ruthenium red. In the collapsed ear, with a marked decrease of proteoglycan in the cartilage, loss of ruthenium red-positive granules was observed in the extracellular matrix. Collagen fibrils in the cartilage appeared to be somewhat increased in number, some of their diameters became slightly greater, and a part were assembled into bundles, occasionally accompanied by periodic crossstriation. Decrease of proteoglycan in the cartilage matrix probably brought about the unmasking and the assembly of collagen fibrils. In one of the experimental animals, collagen fibrous segments of an atypical fibrous long spacing (FLS-)type with symmetrical cross-striation were found around the chondrocytes in the ear cartilage, during the period of recovery. Some kind of the endogenous sulfated carbohydrate may have acted to affect the arrangement of type II collagen or procollagen molecules newly produced by the recovering chondrocytes.Keywords
This publication has 16 references indexed in Scilit:
- The localization of articular cartilage proteoglycan by electron microscopyThe Anatomical Record, 1977
- EPITHELIAL COLLAGENS AND GLYCOSAMINOGLYCANS IN THE EMBRYONIC CORNEAThe Journal of cell biology, 1974
- The Influence of Glycosaminoglycans on the Formation of Fibers from Monomeric Tropocollagen in vitroEuropean Journal of Biochemistry, 1973
- Ruthenium red-positive filaments interconnecting collagen fibrilsJournal of Ultrastructure Research, 1973
- THE ROLE OF MUCOPOLYSACCHARIDES IN VESICLE ARCHITECTURE AND ENDOTHELIAL TRANSPORTThe Journal of cell biology, 1972
- A NEW CHONDRODYSTROPHIC MUTANT IN MICEThe Journal of cell biology, 1971
- STUDIES ON CARTILAGEThe Journal of cell biology, 1960
- PAPAIN-INDUCED CHANGES IN RABBIT CARTILAGEThe Journal of Experimental Medicine, 1958
- THE REMOVAL OF CARTILAGE MATRIX, IN VIVO, BY PAPAINThe Journal of Experimental Medicine, 1958
- REVERSIBLE COLLAPSE OF RABBIT EARS AFTER INTRAVENOUS PAPAIN, AND PREVENTION OF RECOVERY BY CORTISONEThe Journal of Experimental Medicine, 1956