Requirement of endogenous interferon-gamma production for resolution of Listeria monocytogenes infection.
- 1 November 1985
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 82 (21), 7404-7408
- https://doi.org/10.1073/pnas.82.21.7404
Abstract
Peritoneal exudate cells and splenic cells of mice infected with L. monocytogenes show increased production of interferon-.gamma. (IFn-.gamma.) after antigen or mitogen stimulation. When an IFN-.gamma.-specific enzyme-linked immunosorbent assay was used, increased production was first observed 2 days after infection in peritoneal cells and 4 to 6 days after infection in splenic cells. The increased production of IFN-.gamma. correlated with the clearance of Listeria from the peritoneal cavity and spleen. Macrophages derived from mice at these times were activated as evidenced by expression of nonspecific tumoricidal activity against 111In-labeled P815 mastocytoma cells. Injection of neutralizing monoclonal anti-IFN-.gamma. into 1-day-infected mice completely inhibited the generation of activated macrophages. Normal hamster IgG had no effect. In vivo, the monoclonal antibody also abrogated clearance of bacteria from the spleen and peritoneal cavity. Six days after injection of a sublethal dose of Listeria, the peritoneal cavity of control mice treated with normal hamster IgG was devoid of bacteria and the spleen contained less than 103 colony-forming units. However, mice treated with anti-IFN-.gamma. carried more than 8 .times. 106 colony-forming units in either anatomical site at day 6 and exhibited a higher mortality rate. These results indicate that IFN-.gamma. production is required for the in vivo generation of activated macrophages and the clearance of bacteria during Listeria infection.This publication has 33 references indexed in Scilit:
- Monoclonal antibody to murine gamma interferon inhibits lymphokine-induced antiviral and macrophage tumoricidal activities.The Journal of Experimental Medicine, 1984
- Biological and antigenic similarities of murine interferon-gamma and macrophage-activating factor.The Journal of Experimental Medicine, 1984
- Regulation of human peripheral blood monocyte DR antigen expression in vitro by lymphokines and recombinant interferons.JCI Insight, 1984
- Killing of intracellular Leishmania donovani by lymphokine-stimulated human mononuclear phagocytes. Evidence that interferon-gamma is the activating lymphokine.JCI Insight, 1983
- Interferon-γ production byListeria monocytogenes-specific T cells active in cellular antibacterial immunityEuropean Journal of Immunology, 1983
- Enhanced production of murine interferon gamma by T cells generated in response to bacterial infection.The Journal of Experimental Medicine, 1982
- CELLULAR MEDIATORS OF ANTI-LISTERIA IMMUNITY AS AN ENLARGED POPULATION OF SHORT-LIVED, REPLICATING T CELLSThe Journal of Experimental Medicine, 1973
- Cell‐Mediated Immunity to Bacterial Infection in the Mouse. Thymus‐Derived Cells as Effectors of Acquired Resistance to Listeria monocytogenesScandinavian Journal of Immunology, 1972
- REQUIREMENT OF THYMUS (T) LYMPHOCYTES FOR RESISTANCE TO LISTERIOSISThe Journal of Experimental Medicine, 1972
- CELLULAR RESISTANCE TO INFECTIONThe Journal of Experimental Medicine, 1962