Über die Verteilung: der Lipase und Arginase zwischen Zellkern und Protoplasma der Leber. Mit 1 Figur im Text.

Abstract

A new method for the detn. of the distr. of lipase and arginase between protoplasm and cell-nucleus is described. It is based upon the fact that the different histological structures of glycogen-poor livers vary in specific wt. If a sample of finely ground powder of liver, dried in the frozen state, is put into a centrifuge tube with mixtures of CCl4 and C6H6 which are arranged in layers that become heavier from top to bottom, then the liver powder separates into components of different specific wt. which dissolve in the solvent layers of corresponding specific wt. Thus several zones are formed that correspond to more or less homogeneous histological structures. On top the specifically light red blood corpuscles mixed with light protoplasm are collected. The lower layers contain in the following order of their heaviness: 1) protoplasm, 2) protoplasm particles with associated nuclear particles and connective tissue, 3) cell particles, 4) cell particles rich in nuclear material, 5) cell nuclei. With increasing contents of nuclear material the lipase contents of the fractions decrease; the nuclear fraction itself has extraordinarily low lipase value (only 5% of the lipase contents of the protoplasm fraction). This small amt. is apparently due to impurities and the cell nuclei are really free of lipase. The arginase contents in isolated cell nuclei and isolated protoplasm of rabbit livers are about equal.